Ait Benbella Jihane, Housbane Samy, Kadil Youness, Kabbali Fatimaezzahra, Ghicha Ikram, Bazhar Hasnaa, Bousselham Fatiha, Banid Afaf, Hammani Othmane, Louanjli Noureddine, Karkouri Mehdi, Mellouki Abderrahmane, Filali Houda, Aboutaieb Rachid
Laboratory of Sexual and Reproductive Health, Faculty of Medicine and Pharmacy, Hassan II University Casablanca, Casablanca 20360, Morocco.
Department of Medical Informatics, Faculty of Medicine and Pharmacy, Hassan II University Casablanca, Casablanca 20360, Morocco.
Int J Environ Res Public Health. 2025 Mar 20;22(3):454. doi: 10.3390/ijerph22030454.
Cadmium (Cd) is a toxic heavy metal that disrupts spermatogenesis and steroidogenesis due to its long half-life. This study evaluated the impact of recovery alone or with zinc (Zn) supplementation on Cd-induced testicular toxicity. A total of 42 pubertal male Wistar rats were divided into seven groups of six rats each. The control group (1) received NaCl (0.9%). Groups 2, 3, and 4 were treated with Cd 10 μg/kg/d by intraperitoneal injection for 1, 2, and 3 months respectively. Group 5 received Cd for 3 months with a recovery period of 1 month; Group 6 was exposed to Cd for 3 months, followed by a 1-month recovery period combined with Zn supplementation. (0.5 mg/kg/d). The last group was treated with zinc at a dose of 0.5 mg/kg/day for one month. The results showed decreased body weight, testicular and epididymal weight, testicular dimensions, and sperm parameters, along with Cd accumulation in the testes. Cd caused testicular damage and reduced serum testosterone levels, with more pronounced effects in the 3-month treatment group. Recovery alone did not significantly reverse Cd's toxic effects, whereas Zn supplementation mitigated most of the damage. Recovery combined with Zn supplementation was more effective in correcting Cd-induced testicular toxicity than recovery alone.
镉（Cd）是一种有毒重金属，因其半衰期长而会干扰精子发生和类固醇生成。本研究评估了单独恢复或补充锌（Zn）对镉诱导的睾丸毒性的影响。总共42只青春期雄性Wistar大鼠被分为7组，每组6只。对照组（1）接受氯化钠（0.9%）。第2、3和4组分别通过腹腔注射10μg/kg/d的镉，持续1、2和3个月。第5组接受镉处理3个月，恢复期为1个月；第6组暴露于镉3个月，随后是1个月的恢复期并补充锌（0.5mg/kg/d）。最后一组以0.5mg/kg/天的剂量用锌处理1个月。结果显示体重、睾丸和附睾重量、睾丸尺寸以及精子参数下降，同时睾丸中镉蓄积。镉导致睾丸损伤并降低血清睾酮水平，在3个月治疗组中影响更为明显。单独恢复并不能显著逆转镉的毒性作用，而补充锌减轻了大部分损伤。恢复与补充锌相结合在纠正镉诱导的睾丸毒性方面比单独恢复更有效。
