Chen Yinglei, Xiong Changwu, Wan Yingzhi, Sun Mengjun, Zheng Zhong, Liu Dayou, Liao Huilin, Wang Yueqing, Wu Yexu
Hubei Provincial Key Laboratory of Yeast Function, Angel Yeast Co., Ltd., Yichang, China.
Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy (School of Basic Medicine, China Three Gorges University), Yichang, China.
PLoS One. 2025 Apr 16;20(4):e0321650. doi: 10.1371/journal.pone.0321650. eCollection 2025.
Muscle satellite cell (MSC) isolation, proliferation, and differentiation are the basis of cultured meat (CM) technology, which emerged as a sustainable and moral substitute for conventional animal agriculture. Notwithstanding the encouraging future of CM, there are still a lot of obstacles to overcome, like the high expense of cell culture media and the need for fetal bovine serum (FBS). The goal of this work is to determine whether plant-based nitrogen source soy protein hydrolysate (SPH) can improve myogenic differentiation and functional development in MSCs cultured for CM by acting as a serum substitute. We concentrated on how Angel Yeast Company's SPH PU041 affected the C2C12 mouse cell line, a useful model for studying muscle biology and the CM sector. Adding PU041 to cell culture media containing different concentrations of FBS was found to promote C2C12 cell proliferation and elongation, with optimal effects observed at 0.5 g/L. Immunofluorescence and flow cytometry analyses revealed that PU041 up-regulated the protein levels of myosin heavy chain (MyHC) and myogenic differentiation factor 1 (MyoD), key biomarkers in myogenesis. Furthermore, quantitative real-time PCR (qPCR) confirmed the up-regulation of MyHC, MyoD, and myogenin (MyoG) mRNA expression, indicating that PU041 induces myogenic differentiation. The findings suggest that SPH PU041 can potentially be used to reduce the costs associated with CM production as a viable serum substitute, thereby facilitating a more sustainable and ethical approach to food production. However, the precise mechanisms underlying PU041's effects on myogenic differentiation warrant further investigation.
肌肉卫星细胞（MSC）的分离、增殖和分化是细胞培养肉（CM）技术的基础，细胞培养肉作为传统畜牧业的一种可持续且符合道德的替代品而出现。尽管细胞培养肉前景令人鼓舞，但仍有许多障碍需要克服，比如细胞培养基成本高昂以及需要胎牛血清（FBS）。这项工作的目标是确定植物源氮源大豆蛋白水解物（SPH）是否可以作为血清替代品，从而改善用于细胞培养肉的MSC中的肌源性分化和功能发育。我们重点研究了安琪酵母公司的SPH PU041如何影响C2C12小鼠细胞系，这是研究肌肉生物学和细胞培养肉领域的一个有用模型。发现在含有不同浓度FBS的细胞培养基中添加PU041可促进C2C12细胞增殖和伸长，在0.5 g/L时观察到最佳效果。免疫荧光和流式细胞术分析显示，PU041上调了肌球蛋白重链（MyHC）和肌源性分化因子1（MyoD）的蛋白水平，这是肌生成中的关键生物标志物。此外，定量实时PCR（qPCR）证实了MyHC、MyoD和肌细胞生成素（MyoG）mRNA表达上调，表明PU041诱导肌源性分化。研究结果表明，作为一种可行的血清替代品，SPH PU041有可能用于降低细胞培养肉生产的相关成本，从而促进更可持续且符合道德的食品生产方式。然而，PU041对肌源性分化影响的精确机制仍有待进一步研究。
