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旋毛虫排泄分泌抗原对树突状细胞吲哚胺2,3-双加氧酶体外表达的影响

Effects of Trichinella spiralis excretory-secretory antigens on expression of indoleamine 2, 3-dioxygenase on dendritic cells in vitro.

作者信息

Yu Wenhao, Yuan Xuhong, Zhai Peng, Li Xiaoyun, Han Caixia

机构信息

College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.

出版信息

Parasite. 2025;32:26. doi: 10.1051/parasite/2025018. Epub 2025 Apr 16.

DOI:10.1051/parasite/2025018
PMID:40239041
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12002673/
Abstract

Indoleamine 2, 3-dioxygenase (IDO) is a potent immunoenzyme found in dendritic cells (DCs). Research has demonstrated that Trichinella spiralis induces IDO expression in the host immune response through its excretory-secretory (ES) antigens. However, the role of IDO in the immune response to T. spiralis remains unclear. To examine the effects of T. spiralis ES antigens on IDO expression in DCs in vitro, assessments were conducted using qRT-PCR, Western blotting (WB), flow cytometry, and siRNA transfer. The findings indicated that ES antigen stimulation upregulated IDO expression in DCs in vitro. Furthermore, ES antigen significantly enhanced the expression of the proinflammatory cytokines TNF-α and IFN-γ, along with the anti-inflammatory cytokine IL-10, downstream of IDO in DCs. Flow cytometry analysis confirmed that surface molecules CD40, MHC-II, CD80, and CD86 on DCs were upregulated following stimulation with ES antigen and lipopolysaccharide (LPS). Compared to the ES antigen alone, siRNA620 effectively inhibited IDO levels, demonstrating a statistically significant reduction. Continuous stimulation of DCs by ES antigens may lead to immune tolerance through the activation of IDO-mediated inflammation-associated factors. These results suggest that IDO expression in DCs plays a crucial role in T. spiralis infection.

摘要

吲哚胺2,3-双加氧酶（IDO）是一种在树突状细胞（DC）中发现的强效免疫酶。研究表明，旋毛虫通过其排泄-分泌（ES）抗原在宿主免疫反应中诱导IDO表达。然而，IDO在针对旋毛虫的免疫反应中的作用仍不清楚。为了在体外研究旋毛虫ES抗原对DC中IDO表达的影响，使用qRT-PCR、蛋白质免疫印迹法（WB）、流式细胞术和siRNA转染进行了评估。研究结果表明，ES抗原刺激在体外上调了DC中IDO的表达。此外，ES抗原显著增强了DC中IDO下游促炎细胞因子TNF-α和IFN-γ以及抗炎细胞因子IL-10的表达。流式细胞术分析证实，在用ES抗原和脂多糖（LPS）刺激后，DC表面分子CD40、MHC-II、CD80和CD86上调。与单独的ES抗原相比，siRNA620有效抑制了IDO水平，显示出统计学上的显著降低。ES抗原对DC的持续刺激可能通过激活IDO介导的炎症相关因子导致免疫耐受。这些结果表明，DC中IDO的表达在旋毛虫感染中起关键作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b8/12002673/246f8a58f4d4/parasite-32-26-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b8/12002673/d714dc1a64b2/parasite-32-26-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b8/12002673/5eaeec9ae45c/parasite-32-26-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b8/12002673/6c484860c31f/parasite-32-26-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b8/12002673/ffadbc66c096/parasite-32-26-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b8/12002673/8d181764d219/parasite-32-26-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b8/12002673/b0c8b0cbab41/parasite-32-26-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b8/12002673/0fab0c35443c/parasite-32-26-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b8/12002673/246f8a58f4d4/parasite-32-26-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b8/12002673/d714dc1a64b2/parasite-32-26-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b8/12002673/5eaeec9ae45c/parasite-32-26-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b8/12002673/6c484860c31f/parasite-32-26-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b8/12002673/ffadbc66c096/parasite-32-26-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b8/12002673/8d181764d219/parasite-32-26-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b8/12002673/b0c8b0cbab41/parasite-32-26-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b8/12002673/0fab0c35443c/parasite-32-26-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b8/12002673/246f8a58f4d4/parasite-32-26-fig8.jpg

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本文引用的文献

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