Kawayanagi Tomoki, Kawada-Matsuo Miki, Kusaka Satoru, Yasutomi Yoshimasa, Suzuki Yujin, Nishihama Saki, Le Mi Nguyen-Tra, Sugawara Yo, Hisatsune Junzo, Kutsuno Shoko, Asakawa Mikari, Takeshita Toru, Nomura Ryota, Shiba Hideki, Sugai Motoyuki, Komatsuzawa Hitoshi
Department of Biological Endodontics, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
Department of Bacteriology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Kasumi 1-2-3, Hiroshima City, 734-8551, Hiroshima, Japan.
Sci Rep. 2025 Apr 16;15(1):13149. doi: 10.1038/s41598-025-93773-0.
Staphylococcus aureus is a common bacterium that sometimes causes various pyogenic diseases. Methicillin resistant S. aureus (MRSA) infections are particularly difficult to treat. Recently, MRSA has been spreading in the community, so it is important to determine the prevalence of MRSA in the community and to conduct epidemiological studies from genetic and statistical perspectives. In this study, S. aureus/MRSA was isolated from the oral and nasal cavities of 504 dental patients (65 inpatients and 439 outpatients). Sixty-two S. aureus strains and 9 MRSA strains were isolated from the oral cavity, and 112 S. aureus strains and 21 MRSA strains were isolated from the nasal cavity. Multi-locus sequence typing (MLST) analysis showed ST8 was high (18 isolates) among 30 MRSA isolates, whereas among 144 methicillin sensitive isolates, ST15 (25 isolates) and ST8 (20 isolates) were high. Statistical analysis of the patients' clinical status revealed a correlation between oral S. aureus and denture use. Among the 34 patients from whom S. aureus was isolated from both sites, 25 had the same ST, and 23 showed below 40 single-nucleotide polymorphisms which are considered to be identical strains. Our study revealed various properties of S. aureus/MRSA in the oral and nasal cavities as commensals.
金黄色葡萄球菌是一种常见细菌，有时会引发各种化脓性疾病。耐甲氧西林金黄色葡萄球菌（MRSA）感染尤其难以治疗。最近，MRSA已在社区中传播，因此确定社区中MRSA的流行情况并从遗传学和统计学角度开展流行病学研究很重要。在本研究中，从504名牙科患者（65名住院患者和439名门诊患者）的口腔和鼻腔中分离出金黄色葡萄球菌/MRSA。从口腔中分离出62株金黄色葡萄球菌菌株和9株MRSA菌株，从鼻腔中分离出112株金黄色葡萄球菌菌株和21株MRSA菌株。多位点序列分型（MLST）分析显示，在30株MRSA分离株中ST8型居多（18株），而在144株甲氧西林敏感分离株中，ST15型（25株）和ST8型（20株）居多。对患者临床状况的统计分析显示口腔金黄色葡萄球菌与义齿使用之间存在关联。在两个部位均分离出金黄色葡萄球菌的34名患者中，25名具有相同的ST型，23名显示单核苷酸多态性低于40，被认为是同一菌株。我们的研究揭示了口腔和鼻腔中作为共生菌的金黄色葡萄球菌/MRSA的各种特性。
