Campos Joana, Pires Mariana Faria, Sousa Marta, Campos Carla, da Costa Carolina Fernandes Ferreira Alves, Sampaio-Maia Benedita
INEB-Instituto Nacional de Engenharia Biomédica, 4200-135 Porto, Portugal.
i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugal.
Pathogens. 2023 May 26;12(6):765. doi: 10.3390/pathogens12060765.
is both a human commensal and a pathogen, that causes serious nosocomial and community-acquired infections. Despite nostrils being considered its preferred host habitat, the oral cavity has been demonstrated to be an ideal starting point for auto-infection and transmission. The antibiotic resistance assessment of is a priority and is often reported in clinical settings. This study aimed to explore the prevalence and antimicrobial susceptibility of in the oral and nasal cavities of healthy individuals. The participants ( = 101) were subjected to a demographic and clinical background survey, a caries evaluation, and to oral and nasal swabbing. Swabs were cultured in differential/selective media and isolates were identified (MALDI-TOF MS) and tested for antibiotic susceptibility (EUCAST/CLSI). Similar prevalence was found exclusively on nasal (13.9%) or oral (12.0%) habitats, whereas 9.9% of the population were simultaneous nasal and oral carriers. In oro-nasal cavities, similar antibiotic resistance rates (83.3-81.5%), including MDR (20.8-29.6%), were observed. Notably, 60% (6/10) of the simultaneous nasal and oral carriers exhibited different antibiotic resistance profiles between cavities. This study demonstrates the relevance of the oral cavity as an independent colonization site for and as a potential source of antimicrobial resistance, a role which has been widely neglected so far.
既是人体共生菌又是病原菌，可引起严重的医院感染和社区获得性感染。尽管鼻孔被认为是其首选的宿主栖息地，但口腔已被证明是自身感染和传播的理想起始点。对……的抗生素耐药性评估是当务之急，且在临床环境中经常被报告。本研究旨在探讨健康个体口腔和鼻腔中……的流行情况及抗菌药物敏感性。参与者（n = 101）接受了人口统计学和临床背景调查、龋齿评估以及口腔和鼻腔拭子采样。拭子在鉴别/选择性培养基中培养，……分离株被鉴定（基质辅助激光解吸电离飞行时间质谱）并进行抗生素敏感性测试（欧洲抗菌药物敏感性试验委员会/美国临床和实验室标准协会）。仅在鼻腔（13.9%）或口腔（12.0%）栖息地发现了相似的……流行率，而9.9%的人群同时是鼻腔和口腔携带者。在口腔和鼻腔中，观察到相似的抗生素耐药率（83.3 - 81.5%），包括多重耐药率（20.8 - 29.6%）。值得注意的是，60%（6/10）的同时鼻腔和口腔携带者在两个腔之间表现出不同的抗生素耐药谱。本研究证明了口腔作为……独立定植部位以及作为抗菌药物耐药性潜在来源的相关性，这一作用迄今为止一直被广泛忽视。
