Hassan Mervat G, Abdel-Monem Mohamed O, Sleem Al Shaimaa M A, El Awady Mohamed E, Hamed Ahmed A
Botany and Microbiology Department, Faculty of Science, Benha Univ., Benha, Egypt.
Microbial Biotechnology Department, National Research Centre, El-Buhouth St. 33, Dokki-Cairo, 12622, Egypt.
BMC Microbiol. 2025 Apr 16;25(1):219. doi: 10.1186/s12866-025-03912-w.
The genus Streptomyces has been recently proven to be a valuable and rich source of producing several bioactive compounds with substantial biological activity and applications in many fields such as medicine, environmental science, food industries, and agronomy. This study highlights the importance of Streptomyces as an antimicrobial, antibiofilm, and anticancer. Out of the 75 actinobacteria isolated from both marine and soil habitats, one isolate, HG2, was selected based on its potent antimicrobial activity. The isolate has been identified morphologically by studying colony and spore chain morphology using TEM and genetically by sequencing their 16 sr RNA gene as Streptomyces sp. 22SH with Accession number OK326829.1. Bioassay-guided fractionation of the Streptomyces sp. 22SH crude extract led to the isolation and purification of Cis-9-Octadecenoic. Biological evaluation including antimicrobial and antibiofilm activity of the crude and purified compound was performed on four clinical microbes (S. aureus ATCC6538-P, B. subtilis ATCC6633, and P. aeruginosa ATCC27853). The compound showed the ability to eradicate the biofilm formation by the tested pathogens. Additionally, the antitumor activity was assessed, and the compound showed a cytotoxic effect against liver carcinoma and breast cancer cells, with IC50 values of 17.48 ± 0.94 and 88.73 ± 4.78 µg/ml, respectively. While it displayed anti-topoisomerase activity with an IC of 0.65 ± 0.023 µg/ml. Furthermore, the compound's ADME-related physicochemical features and docking analysis were investigated.
最近已证明，链霉菌属是生产多种具有重要生物活性的生物活性化合物的宝贵且丰富的来源，这些化合物在医学、环境科学、食品工业和农学等许多领域都有应用。本研究强调了链霉菌作为抗菌、抗生物膜和抗癌剂的重要性。在从海洋和土壤生境中分离出的75株放线菌中，根据其强大的抗菌活性选择了一株HG2菌株。通过透射电子显微镜（TEM）研究菌落和孢子链形态，从形态学上对该菌株进行了鉴定，并通过对其16S rRNA基因进行测序，从基因上鉴定其为链霉菌属22SH，登录号为OK326829.1。对链霉菌属22SH粗提物进行生物测定导向的分级分离，导致顺-9-十八碳烯酸的分离和纯化。对四种临床微生物（金黄色葡萄球菌ATCC6538-P、枯草芽孢杆菌ATCC6633和铜绿假单胞菌ATCC27853）进行了粗提物和纯化化合物的抗菌和抗生物膜活性等生物学评价。该化合物显示出消除受试病原体生物膜形成的能力。此外，还评估了其抗肿瘤活性，该化合物对肝癌和乳腺癌细胞显示出细胞毒性作用，IC50值分别为17.48±0.94和88.73±4.78μg/ml。同时，它显示出抗拓扑异构酶活性，IC值为0.65±0.023μg/ml。此外，还研究了该化合物与ADME相关的物理化学特征和对接分析。
