Zhang Zehua, Gao Zhigang, Jia Zhenwei
College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, China.
Anim Biosci. 2025 Aug;38(8):1644-1656. doi: 10.5713/ab.24.0847. Epub 2025 Apr 11.
The present study evaluated the beneficial effects of pyrroloquinoline quinone (PQQ) on in vitro maturation (IVM) of porcine oocyte and subsequent early embryo development.
Porcine cumulus oocyte complexes were cultured in IVM medium with supplementation of 0, 200, 400, 800 or 1600 nM PQQ for 42 h. We first examined cumulus expansion index (CEI) and the rate of oocyte nuclear maturation. Then, we assessed oocyte mitochondrial function, oxidative stress levels, lipid metabolism and subsequent embryonic development.
PQQ (800 nM) supplementation significantly increased CEI and the nuclear maturation rate of oocytes following IVM. Additionally, oocysts supplemented with 800 nM of PQQ showed significantly increased mitochondrial content, mitochondrial membrane potential, activity, and mRNA expression levels of genes associated with mitochondrial biogenesis (PGC-1a, NRF1, NRF2 and TFAM). PQQ significantly reduced the levels of reactive oxygen species, lipid drop-lets, and fatty acids content, while enhancing the mRNA expression levels of genes related to antioxidant activity (SOD1, SOD2, GPX and CAT), lipolysis (ATGL and HSL) and β-oxidation (CPT1B and CPT2) in porcine oocytes. PQQ (800 nM) supplementation significantly increased cleavage rate, blastocyst formation rate, and total blastocyst cell numbers following partheno-genetic activation.
PQQ supplementation during IVM positively influences porcine oocyte maturation and subse-quent embryonic development by enhancing mitochondrial function and lipid metabolism and alleviating oxidative stress.
本研究评估了吡咯喹啉醌（PQQ）对猪卵母细胞体外成熟（IVM）及随后早期胚胎发育的有益作用。
将猪卵丘卵母细胞复合体在添加0、200、400、800或1600 nM PQQ的IVM培养基中培养42小时。我们首先检测卵丘扩展指数（CEI）和卵母细胞核成熟率。然后，评估卵母细胞的线粒体功能、氧化应激水平、脂质代谢及随后的胚胎发育。
添加800 nM PQQ显著提高了IVM后卵母细胞的CEI和核成熟率。此外，添加800 nM PQQ的卵母细胞显示线粒体含量、线粒体膜电位、活性以及与线粒体生物发生相关基因（PGC-1α、NRF1、NRF2和TFAM）的mRNA表达水平显著增加。PQQ显著降低了活性氧、脂滴和脂肪酸含量水平，同时提高了猪卵母细胞中与抗氧化活性（SOD1、SOD2、GPX和CAT）、脂解（ATGL和HSL）及β-氧化（CPT1B和CPT2）相关基因的mRNA表达水平。添加800 nM PQQ显著提高了孤雌激活后的卵裂率、囊胚形成率和囊胚总细胞数。
IVM期间添加PQQ通过增强线粒体功能和脂质代谢以及减轻氧化应激，对猪卵母细胞成熟和随后的胚胎发育产生积极影响。
