Shaanxi Stem Cell Engineering and Technology Research Center, College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, China.
Theriogenology. 2022 Oct 15;192:89-96. doi: 10.1016/j.theriogenology.2022.08.028. Epub 2022 Aug 29.
An optimal lipid droplet (LD) content is essential for successful mammalian embryonic development. Salidroside (SAL) is a traditional Chinese medicine and one of the important active components of the Rhodiola plant. SAL possesses antioxidative, anti-aging, and cardiovascular properties. Here, we studied the effects of SAL on in vitro maturation (IVM) of porcine oocytes and the subsequent embryonic development after parthenogenetic activation (PA). We found that 100 μM of SAL had no effect on the extrusion rate of the first polar body of porcine oocytes but significantly improved the subsequent blastocyst formation rate and embryo quality. Our study further revealed that SAL treatment altered the morphology, increased the lipid content in oocytes, increased mitochondrial number. Further analysis revealed that SAL upregulated the expression of genes related to lipid metabolism (FASN, FADS1, HSL, and CPT1a) and the mitochondria function-related genes (PGC-1α). These results suggest that SAL supplementation enhances oocyte maturation and subsequent embryonic development by promoting lipid metabolism, providing the necessary energy for the aforementioned processes.
最佳的脂滴（LD）含量对于哺乳动物胚胎的成功发育至关重要。红景天苷（SAL）是一种中药，也是红景天植物的重要活性成分之一。SAL 具有抗氧化、抗衰老和心血管保护作用。在这里，我们研究了 SAL 对猪卵母细胞体外成熟（IVM）和随后的孤雌激活（PA）后胚胎发育的影响。我们发现，100μM 的 SAL 对猪卵母细胞第一极体的挤出率没有影响，但显著提高了随后的囊胚形成率和胚胎质量。我们的研究进一步表明，SAL 处理改变了卵母细胞的形态，增加了脂滴的含量，增加了线粒体的数量。进一步的分析表明，SAL 上调了与脂代谢（FASN、FADS1、HSL 和 CPT1a）和线粒体功能相关的基因（PGC-1α）的表达。这些结果表明，SAL 补充通过促进脂代谢增强卵母细胞成熟和随后的胚胎发育，为上述过程提供必要的能量。
