Sitarek Przemysław, Owczarek Monika, Kowalczyk Tomasz, Kukula-Koch Wirginia, Lasoń-Rydel Magdalena, Herczyńska Lucyna
Department of Medical Biology, Medical University of Lodz, Muszyńskiego 1, 90-151 Lodz, Poland.
Łukasiewicz Research Network-Lodz Institute of Technology, Skłodowskiej-Curie 19/27, 90-570 Lodz, Poland.
Int J Mol Sci. 2025 Mar 27;26(7):3066. doi: 10.3390/ijms26073066.
The dynamic development of various branches of medicine and pharmacy, along with the emergence of new preventive and alternative therapies for various diseases, creates opportunities for new solutions utilizing carriers of active substances. Their therapeutic effect may occur through direct contact with skin lesions or indirectly, where medicinal substances penetrate the capillary network in the deeper layers of the skin and reach the bloodstream. The aim of the research was to obtain carriers with a matrix consisting of two renewable-source polymers (chitosan and ethylcellulose) and a core material derived from green leaf extract (GBE). The obtained ethylcellulose microcapsules with encapsulated chitosan nanoparticles with extract {Et[Ch(GB)NP]} were characterized with respect to size, shape, surface morphology (SEM microscopy), and active substance release kinetics (UV-VIS and mathematical release models). The kinetics of active substance release were analyzed using UV-VIS spectroscopy and mathematical release models. The released active components were assessed microbiologically for activity against six bacterial strains and two fungal strains, as well as chromatographically using HPLC-ESI-QTOF-MS/MS fingerprinting. The microcapsules with a dual polymer layer exhibited a slow release of the core material, which demonstrated microbiological activity. The strongest antimicrobial effects were observed against and , with a minimum inhibitory concentration (MIC) of 410 µg/mL. The release of the core material from the double-layer polymer structures was more efficient in a physiological saline environment, with the best fit for the extract release kinetics following a zero-order model (regression coefficient R = 0.9939). The obtained microcapsules with a dual polymer layer show great potential for therapeutic applications in the medical industry. Their controlled release properties and antibacterial effectiveness make them a promising carrier for active substances in modern therapies.
医学和药学各分支的动态发展，以及针对各种疾病的新预防和替代疗法的出现，为利用活性物质载体的新解决方案创造了机会。它们的治疗效果可能通过与皮肤损伤直接接触或间接发生，即药用物质穿透皮肤深层的毛细血管网络并进入血液循环。本研究的目的是获得一种载体，其基质由两种可再生源聚合物（壳聚糖和乙基纤维素）和一种源自绿叶提取物（GBE）的核心材料组成。对所得的包封有壳聚糖纳米颗粒与提取物的乙基纤维素微胶囊{Et[Ch(GB)NP]}进行了尺寸、形状、表面形态（扫描电子显微镜）和活性物质释放动力学（紫外可见光谱和数学释放模型）方面的表征。使用紫外可见光谱和数学释放模型分析活性物质的释放动力学。对释放的活性成分进行微生物学评估，以检测其对六种细菌菌株和两种真菌菌株的活性，并使用高效液相色谱-电喷雾-四极杆飞行时间串联质谱指纹图谱进行色谱分析。具有双层聚合物层的微胶囊显示出核心材料的缓慢释放，且具有微生物活性。对[具体细菌名称1]和[具体细菌名称2]观察到最强的抗菌作用，最低抑菌浓度（MIC）为410µg/mL。核心材料从双层聚合物结构中的释放在生理盐水环境中更有效，提取物释放动力学最符合零级模型（回归系数R = 0.9939）。所得的具有双层聚合物层的微胶囊在医疗行业的治疗应用中显示出巨大潜力。它们的控释特性和抗菌有效性使其成为现代疗法中活性物质的有前途的载体。
