Chen Jiahao, Liu Yihao, Zhao Hongbo, Xu Jianmei, Zheng Peng, Liu Shaoqun, Sun Binmei
College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Int J Mol Sci. 2025 Apr 1;26(7):3253. doi: 10.3390/ijms26073253.
Tea is one of the world's major non-alcoholic beverages, popular for its health benefits and flavor. Purple-bud tea is particularly rich in anthocyanins, the concentration of which varies depending on the tea cultivar and cultivation conditions. While the genetic regulation of anthocyanin accumulation is well understood, the impact of environmental factors, such as light, on anthocyanin synthesis is less documented. In this study, we analyzed the anthocyanin content and the expression levels of , , and , under different light intensities and durations. The expression of both and was significantly induced by light, with an intensity of 8000 lx particularly effective in promoting anthocyanin accumulation. Furthermore, we explored the effect of shading on anthocyanin content, finding that fifty percent shading reduced anthocyanin content by nearly half. Finally, dual-luciferase reporter assays and yeast one-hybrid assays confirmed the direct regulation of on . These findings offer insights into the regulatory mechanisms underlying light-induced anthocyanin biosynthesis in tea plants and suggest a potential method for controlling anthocyanin accumulation in tea production.
茶是世界上主要的非酒精饮料之一，因其对健康有益且味道可口而广受欢迎。紫芽茶尤其富含花青素，其含量因茶叶品种和种植条件而异。虽然花青素积累的遗传调控已得到充分了解，但光照等环境因素对花青素合成的影响记录较少。在本研究中，我们分析了不同光照强度和时长下花青素含量以及相关基因的表达水平。两个相关基因的表达均受到光照显著诱导，8000勒克斯的光照强度对促进花青素积累尤为有效。此外，我们探究了遮荫对花青素含量的影响，发现50%的遮荫使花青素含量降低了近一半。最后，双荧光素酶报告基因检测和酵母单杂交检测证实了某基因对另一基因的直接调控作用。这些发现为茶树中光诱导花青素生物合成的调控机制提供了见解，并为控制茶叶生产中花青素积累提供了潜在方法。
