Hisamoto Kazufumi, Toyama Shogo, Okubo Naoki, Kamada Yoichiro, Nakagawa Shuji, Arai Yuji, Inoue Atsuo, Mazda Osam, Takahashi Kenji
Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan.
Department of Sports and Para-Sports Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan.
Int J Mol Sci. 2025 Apr 2;26(7):3294. doi: 10.3390/ijms26073294.
We investigated the effects of belt electrode-skeletal muscle electrical stimulation (B-SES) on muscle atrophy in collagen-induced arthritis (CIA) rats. Twenty-eight 8-week-old male Dark Agouti rats were immunized with type II collagen and Freund's incomplete adjuvant (day 0). From days 14 to 28, 18 rats received B-SES (50 Hz) four times only on the right hindlimb (STIM), while the contralateral left hindlimb remained unstimulated. Both hindlimbs of 10 untreated CIA rats were defined as controls (CONT). Paw volume was measured every other day. On day 28, the muscle weight, histology, and gene expression of the soleus and extensor digitorum longus (EDL) were analyzed. B-SES did not worsen paw volume throughout the experimental period. Compared with CONT, the muscle weight and fiber cross-sectional area of the soleus were higher in STIM. The expression of muscle degradation markers (atrogin-1 and MuRF-1) in the soleus and EDL was lower in the STIM group than that in the CONT group. In contrast, B-SES did not significantly affect the expression of muscle synthesis (Eif4e and p70S6K) and mitochondrial (PGC-1α) markers. B-SES prevents muscle atrophy in CIA rats by reducing muscle degradation without exacerbating arthritis, demonstrating its promising potential as an intervention for RA-induced muscle atrophy.
我们研究了带状电极-骨骼肌电刺激（B-SES）对胶原诱导性关节炎（CIA）大鼠肌肉萎缩的影响。28只8周龄雄性黑褐大鼠用II型胶原和弗氏不完全佐剂进行免疫（第0天）。从第14天到第28天，18只大鼠仅对右后肢进行4次B-SES（50Hz）刺激（刺激组），而对侧左后肢不进行刺激。10只未治疗的CIA大鼠的双侧后肢定义为对照组（对照组）。每隔一天测量爪体积。在第28天，分析比目鱼肌和趾长伸肌（EDL）的肌肉重量、组织学和基因表达。在整个实验期间，B-SES并未使爪体积恶化。与对照组相比，刺激组比目鱼肌的肌肉重量和纤维横截面积更高。刺激组比目鱼肌和EDL中肌肉降解标志物（atrogin-1和MuRF-1）的表达低于对照组。相反，B-SES对肌肉合成（Eif4e和p70S6K）和线粒体（PGC-1α）标志物的表达没有显著影响。B-SES通过减少肌肉降解来预防CIA大鼠的肌肉萎缩，且不会加重关节炎，表明其作为类风湿关节炎诱导的肌肉萎缩干预措施具有广阔前景。
