Zhu Kang, Li Zhanyuan, Cao Jingjing, Cao Yixi, Wang Jimei, Wang Shiyu, Chen Ling, Zhou Huiqin, Huang Wei, Zou Hanxun, Li Qunsheng, Mu Jing, Song Jibin
State Key Laboratory of Chemical Resource Engineering, College of Chemistry, College of Chemical Engineering, Beijing University of Chemical Technology, Beijing, 100029, P. R. China.
Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Department of Radiation Oncology, Jinan, 250117, P. R. China.
Adv Sci (Weinh). 2025 Jun;12(23):e2417828. doi: 10.1002/advs.202417828. Epub 2025 Apr 17.
The efficacy of radiotherapy (RT) is often limited by insufficient tumor selectivity and suboptimal therapeutic responses. To overcome these problems, a new kind of selenium-doped Ag/AgS Janus nanoparticles (Ag/AgSeS JNPs) is presented as radio-responsive molecular probes for precise tumor imaging and enhanced radiosensitization. By adjusting the selenium precursor input, heterojunction nanoparticles with tunable doping ratios are synthesized, optimizing X-ray absorption and energy storage properties. Upon X-ray irradiation, the Ag/AgSeS JNPs interact with overexpressed hydrogen peroxide (HO) in tumor cells, generating highly toxic hydroxyl radicals (·OH), which effectively induce tumor cell apoptosis. Additionally, Selenium incorporation improves electron-hole pair separation efficiency and enhances the photocurrent response, promoting increased electron transfer and ·OH generation, thus amplifying reactive oxygen species (ROS) production and enhancing radiosensitization. Furthermore, the fluorescence "OFF-ON" mechanism, triggered by HO-induced etching of silver allows real-time monitoring of HO levels via the second near-infrared window (NIR-II) fluorescence (FL) imaging "Turn On", which delineates tumor boundaries for precise RT and reduce side effects to normal tissue. This dual-functional platform not only enables real-time tracking but also enhances therapeutic outcomes, offering a promising approach to precision cancer treatment.
放射疗法（RT）的疗效常常受到肿瘤选择性不足和治疗反应欠佳的限制。为克服这些问题，一种新型的硒掺杂Ag/AgS Janus纳米颗粒（Ag/AgSeS JNPs）被提出作为放射响应性分子探针，用于精确的肿瘤成像和增强放射增敏作用。通过调整硒前驱体的输入量，合成了具有可调掺杂比的异质结纳米颗粒，优化了X射线吸收和能量存储特性。在X射线照射下，Ag/AgSeS JNPs与肿瘤细胞中过表达的过氧化氢（HO）相互作用，产生剧毒的羟基自由基（·OH），有效诱导肿瘤细胞凋亡。此外，掺入硒提高了电子-空穴对的分离效率，增强了光电流响应，促进了电子转移增加和·OH生成，从而放大了活性氧（ROS）的产生并增强了放射增敏作用。此外，由HO诱导的银蚀刻触发的荧光“关闭-开启”机制，允许通过第二个近红外窗口（NIR-II）荧光（FL）成像“开启”实时监测HO水平，这描绘了肿瘤边界以进行精确的RT并减少对正常组织的副作用。这个双功能平台不仅能够进行实时跟踪，还能提高治疗效果，为精确癌症治疗提供了一种有前景的方法。
