Chaĭkovskiĭ V S, Basharina O B, Zorina A D, Korneva I A, Rogozkin V A
Vopr Med Khim. 1985 May-Jun;31(3):120-4.
A radioimmunoassay (RIA) of androstenedione in biological fluids was developed. The sensitivity of the method was 30 pg/ml. The efficiency of the standard detection in blood and saliva was 98.2%. The intra- and interassay variations were 5.6% and 12,7%, respectively. Specificity pf the antiserum was determined in cross reactions using 17 steroids. The specificity depended upon the method used, the duration of immunization and the quantity of the antigen. Mathematic methods were used for definition of optimal values of the main parameters and conditions for RIA. 60 different regimes were investigated. The influence of regular physical exercises on androstenedione content in blood and saliva was investigated in 100 athletes. Physical exercises increased androstenedione concentration in men and women blood and saliva. Concentration of androstenedione was increased 1.3- and 2.2-fold in men blood and saliva, respectively, the concentration of androstenedione was increased 1.6-fold in women blood.
开发了一种生物体液中雄烯二酮的放射免疫测定法（RIA）。该方法的灵敏度为30 pg/ml。血液和唾液中标准检测的效率为98.2%。批内和批间变异分别为5.6%和12.7%。使用17种类固醇通过交叉反应确定抗血清的特异性。特异性取决于所用方法、免疫持续时间和抗原量。采用数学方法确定RIA主要参数的最佳值和条件。研究了60种不同方案。对100名运动员进行了规律体育锻炼对血液和唾液中雄烯二酮含量影响的研究。体育锻炼使男性和女性血液及唾液中的雄烯二酮浓度升高。男性血液和唾液中雄烯二酮浓度分别升高1.3倍和2.2倍，女性血液中雄烯二酮浓度升高1.6倍。
