[Radioimmunologic determination of androstenedione and the effect of physical training on its levels in human blood and saliva].

A radioimmunoassay (RIA) of androstenedione in biological fluids was developed. The sensitivity of the method was 30 pg/ml. The efficiency of the standard detection in blood and saliva was 98.2%. The intra- and interassay variations were 5.6% and 12,7%, respectively. Specificity pf the antiserum was determined in cross reactions using 17 steroids. The specificity depended upon the method used, the duration of immunization and the quantity of the antigen. Mathematic methods were used for definition of optimal values of the main parameters and conditions for RIA. 60 different regimes were investigated. The influence of regular physical exercises on androstenedione content in blood and saliva was investigated in 100 athletes. Physical exercises increased androstenedione concentration in men and women blood and saliva. Concentration of androstenedione was increased 1.3- and 2.2-fold in men blood and saliva, respectively, the concentration of androstenedione was increased 1.6-fold in women blood.