Kalliampakou Katerina I, Athanasopoulou Evmorfia, Spanou Aikaterini, Flemetakis Emmanouil, Tsironi Theofania
Laboratory of Food Process Engineering, Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, Athens, 11855, Greece.
Laboratory of Molecular Biology, Department of Biotechnology, Agricultural University of Athens, Iera Odos 75, Athens, 11855, Greece.
Sci Rep. 2025 Apr 17;15(1):13304. doi: 10.1038/s41598-025-98163-0.
Edible raw materials have gained attention as sustainable food packaging films, which are often considered a priori safe for human consumption. However, cytotoxicity issues may arise due to the incorporation of additives or modifications of film functionality during the manufacturing process. This study introduces an integrated methodology for the evaluation of potential migration of cytotoxic substances from materials used for the development of conventional and biodegradable food packaging. Carboxymethyl cellulose (CMC) and sodium alginate (SA) were tested as raw materials of an edible (CMC-SA) film, while a low-density-polyethylene (LDPE) film was tested as a conventional material. The CMC-SA film exhibited higher water vapor transmission rate and water vapor permeability, and lower hydrophobicity compared to LDPE (WVTR=1457.87 vs. WVTR=3.43 g×m×day, WVP=43.24 vs. WVP=0.0048 g×m×mm×day×kPa and CA=52.05 vs. CA=94.28°, respectively). An analytical protocol based on EU Regulation 10/2011 was introduced, to evaluate the potential migration of cytotoxic packaging substances into food simulants, using different human cells. Caco2 cells were used to simulate human intestine, whereas Huh7 and Immortalized Human Hepatocytes (IHH) cells simulated human liver. Cell viability assays and gene expression results indicated that substances migrating from the tested packaging materials neither produced cell cytotoxicity, nor induced oxidative stress to Caco2 cells.
可食用原材料作为可持续食品包装薄膜受到了关注，它们通常被认为对人类食用具有先天安全性。然而，在制造过程中，由于添加剂的加入或薄膜功能的改变，可能会出现细胞毒性问题。本研究介绍了一种综合方法，用于评估传统和可生物降解食品包装材料中细胞毒性物质的潜在迁移。羧甲基纤维素（CMC）和海藻酸钠（SA）作为可食用（CMC-SA）薄膜的原材料进行了测试，而低密度聚乙烯（LDPE）薄膜作为传统材料进行了测试。与LDPE相比，CMC-SA薄膜表现出更高的水蒸气透过率和水蒸气渗透率，以及更低的疏水性（WVTR分别为1457.87和3.43 g×m×天，WVP分别为43.24和0.0048 g×m×mm×天×kPa，CA分别为52.05和94.28°）。引入了基于欧盟法规10/2011的分析方案，以评估细胞毒性包装物质向食品模拟物中的潜在迁移，使用不同的人类细胞。Caco2细胞用于模拟人类肠道，而Huh7和永生化人类肝细胞（IHH）细胞模拟人类肝脏。细胞活力测定和基因表达结果表明，从测试包装材料迁移出的物质既不会产生细胞毒性，也不会对Caco2细胞诱导氧化应激。
