• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

万氏莪术根茎的抗炎活性及通过生物活性导向分离与网络药理学相结合寻找其生物活性标志物

Anti-inflammatory activity of Curcuma wanenlueanga Saensouk, Thomudtha & Boonma rhizomes and the search for its bioactive markers by harmonizing bioassay-guided isolation and network pharmacology.

作者信息

Thongphichai Wisuwat, Hasriadi Hasriadi, Wasana Peththa Wadu Dasuni, Jayashan Sanith Sri, Sritularak Boonchoo, Towiwat Pasarapa, Sukrong Suchada

机构信息

Center of Excellence in DNA Barcoding of Thai Medicinal Plants, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.

Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.

出版信息

BMC Complement Med Ther. 2025 Apr 17;25(1):143. doi: 10.1186/s12906-025-04884-0.

DOI:10.1186/s12906-025-04884-0
PMID:40247235
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12004807/
Abstract

BACKGROUND

Rhizomes of Curcuma wanenlueanga Saensouk, Thomudtha & Boonma have been used in Thai traditional medicine and are included as an ingredient in a Thai traditional liniment formula listed by the Ministry of Public Health of Thailand for treating symptoms related to joints and muscle inflammation. However, anti-inflammatory activity and bioactive constituents of C. wanenlueanga have not yet been investigated. Thus, this study aimed to investigate the anti-inflammatory activity and underlying mechanism of C. wanenlueanga rhizome extract and its responsible bioactive components.

METHODS

The fractionation of bioactive compounds from C. wanenlueanga extract was guided by antioxidant activity on DPPH and Griess assays, and anti-inflammatory activity on LPS-induced RAW 264.7 cells. The biological activities of isolated compounds were first predicted by network pharmacology and further confirmed in cell-based assay with LPS-induced RAW 264.7 cells and enzyme-linked immunosorbent assay (ELISA) of tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6).

RESULTS

The ethanolic extract of C. wanenlueanga rhizomes was proved to show anti-inflammatory activity on LPS-induced RAW 264.7 cells. Three curcuminoids including curcumin (1), demethoxycurcumin (2), dihydrodemethoxycurcumin (3), and two sesquiterpenoids, namely curcumenone (4), and zedoarondiol (5) were separated from anti-inflammatory fractions of C. wanenlueanga extract. The anti-inflammatory activity of these compounds to attenuate the productions of TNF-α and IL-6 was predicted by pharmacological network. The inflammatory assays, including the Griess assay for NO and ELISA for TNF-α and IL-6, confirmed that all isolated compounds reduced the production of these inflammatory mediators.

CONCLUSION

The present study shows the accordance between the results from pharmacological network and cell-based assays, which indicate the anti-inflammatory activity of C. wanenlueanga rhizomes and their bioactive constituents. This suggests the potential of bioactive compounds 1-5 to be used for quality assessment of C. wanenlueanga extract in the development of herbal products.

摘要

背景

温郁金（Curcuma wanenlueanga Saensouk、Thomudtha & Boonma）的根茎已用于泰国传统医学，并被列为泰国公共卫生部列出的一种泰国传统擦剂配方的成分，用于治疗与关节和肌肉炎症相关的症状。然而，温郁金的抗炎活性和生物活性成分尚未得到研究。因此，本研究旨在探讨温郁金根茎提取物的抗炎活性及其潜在机制，以及其起作用的生物活性成分。

方法

以对二苯基苦味酰基自由基（DPPH）和格里斯（Griess）试验的抗氧化活性以及对脂多糖（LPS）诱导的RAW 264.7细胞的抗炎活性为指导，对温郁金提取物中的生物活性化合物进行分离。首先通过网络药理学预测分离出的化合物的生物学活性，并在基于细胞的LPS诱导的RAW 264.7细胞试验以及肿瘤坏死因子α（TNF-α）和白细胞介素-6（IL-6）的酶联免疫吸附测定（ELISA）中进一步证实。

结果

温郁金根茎的乙醇提取物被证明对LPS诱导的RAW 264.7细胞具有抗炎活性。从温郁金提取物的抗炎部分中分离出三种姜黄素类化合物，即姜黄素（1）、去甲氧基姜黄素（2）、二氢去甲氧基姜黄素（3），以及两种倍半萜类化合物，即莪术二酮（4）和莪术二醇（5）。通过药理学网络预测了这些化合物减弱TNF-α和IL-6产生的抗炎活性。包括用于检测一氧化氮（NO）的格里斯试验以及用于检测TNF-α和IL-6的ELISA在内的炎症试验证实，所有分离出的化合物均降低了这些炎症介质的产生。

结论

本研究表明药理学网络和基于细胞的试验结果一致，这表明温郁金根茎及其生物活性成分具有抗炎活性。这表明生物活性化合物1 - 5在草药产品开发中用于温郁金提取物质量评估的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/870b/12004807/934ced73942f/12906_2025_4884_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/870b/12004807/5b3caf83ea07/12906_2025_4884_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/870b/12004807/d194db331890/12906_2025_4884_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/870b/12004807/070ca23912e5/12906_2025_4884_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/870b/12004807/0bb6ede1b2c6/12906_2025_4884_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/870b/12004807/6a1d94b408f1/12906_2025_4884_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/870b/12004807/4c57678575f8/12906_2025_4884_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/870b/12004807/934ced73942f/12906_2025_4884_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/870b/12004807/5b3caf83ea07/12906_2025_4884_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/870b/12004807/d194db331890/12906_2025_4884_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/870b/12004807/070ca23912e5/12906_2025_4884_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/870b/12004807/0bb6ede1b2c6/12906_2025_4884_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/870b/12004807/6a1d94b408f1/12906_2025_4884_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/870b/12004807/4c57678575f8/12906_2025_4884_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/870b/12004807/934ced73942f/12906_2025_4884_Fig7_HTML.jpg

相似文献

1
Anti-inflammatory activity of Curcuma wanenlueanga Saensouk, Thomudtha & Boonma rhizomes and the search for its bioactive markers by harmonizing bioassay-guided isolation and network pharmacology.万氏莪术根茎的抗炎活性及通过生物活性导向分离与网络药理学相结合寻找其生物活性标志物
BMC Complement Med Ther. 2025 Apr 17;25(1):143. doi: 10.1186/s12906-025-04884-0.
2
Anti-inflammatory and antinociceptive effects of Curcuma kwangsiensis and its bioactive terpenoids in vivo and in vitro.广西莪术及其生物活性三萜类化合物的体内外抗炎和镇痛作用。
J Ethnopharmacol. 2020 Sep 15;259:112935. doi: 10.1016/j.jep.2020.112935. Epub 2020 May 6.
3
Bioactivity-based analysis and chemical characterization of anti-inflammatory compounds from Curcuma zedoaria rhizomes using LPS-stimulated RAW264.7 cells.基于生物活性分析和化学表征的姜黄根茎中抗炎化合物的研究，采用 LPS 刺激 RAW264.7 细胞。
Bioorg Chem. 2019 Feb;82:26-32. doi: 10.1016/j.bioorg.2018.09.027. Epub 2018 Sep 20.
4
Bioassay-guided study of the anti-inflammatory effect of Anoectochilus burmannicus ethanolic extract in RAW 264.7 cells.基于生物测定的研究：拳参正丁醇提取物对 RAW 264.7 细胞的抗炎作用。
J Ethnopharmacol. 2021 Nov 15;280:114452. doi: 10.1016/j.jep.2021.114452. Epub 2021 Jul 24.
5
In Vitro Anti-Inflammatory, Anti-Oxidant, and Cytotoxic Activities of Four Species and the Isolation of Compounds from Rhizome.四种植物的体外抗炎、抗氧化和细胞毒性活性及根茎中化合物的分离
Biomolecules. 2020 May 21;10(5):799. doi: 10.3390/biom10050799.
6
Antiinflammation constituents from Curcuma zedoaroides.莪术抗炎成分。
Phytother Res. 2018 Nov;32(11):2312-2320. doi: 10.1002/ptr.6173. Epub 2018 Aug 15.
7
Evaluation of the in vitro and in vivo anti-inflammatory activity of Gymnosporia montana (Roth). Benth leaves.评价采木（Roth）。宾氏 Gymnosporia montana 叶的体外和体内抗炎活性。
J Ethnopharmacol. 2022 Oct 28;297:115539. doi: 10.1016/j.jep.2022.115539. Epub 2022 Jul 14.
8
Identification of galangin as the bioactive compound from Alpinia calcarata (Haw.) Roscoe rhizomes to inhibit IRAK-1/ MAPK/ NF-κB p65 and JAK-1 signaling in LPS stimulated RAW 264.7 cells.鉴定高良姜素为益智（Alpinia calcarata（Haw.）Roscoe）根茎中抑制脂多糖刺激 RAW 264.7 细胞中 IRAK-1/MAPK/NF-κB p65 和 JAK-1 信号通路的活性化合物。
J Ethnopharmacol. 2022 Apr 24;288:114975. doi: 10.1016/j.jep.2022.114975. Epub 2022 Jan 11.
9
Isolation and identification of the components in Cybister chinensis Motschulsky against inflammation and their mechanisms of action based on network pharmacology and molecular docking.基于网络药理学和分子对接技术研究中华虎凤蝶抗炎成分的分离鉴定及其作用机制。
J Ethnopharmacol. 2022 Mar 1;285:114851. doi: 10.1016/j.jep.2021.114851. Epub 2021 Nov 19.
10
Curcuma latifolia Roscoe extract reverses inflammatory pain in mice and offers a favorable CNS safety profile.蓬莪术提取物可逆转小鼠的炎症性疼痛，并呈现出有利的中枢神经系统安全性特征。
J Ethnopharmacol. 2024 Jan 10;318(Pt A):116877. doi: 10.1016/j.jep.2023.116877. Epub 2023 Jul 12.

本文引用的文献

1
Immunomodulatory roles of metalloproteinases in rheumatoid arthritis.金属蛋白酶在类风湿性关节炎中的免疫调节作用
Front Pharmacol. 2023 Nov 15;14:1285455. doi: 10.3389/fphar.2023.1285455. eCollection 2023.
2
Network pharmacology: a bright guiding light on the way to explore the personalized precise medication of traditional Chinese medicine.网络药理学：探索中药个性化精准用药道路上的一盏明灯。
Chin Med. 2023 Nov 8;18(1):146. doi: 10.1186/s13020-023-00853-2.
3
Antichilblain Components in Eggplant Based on Network Pharmacology and Biological Evaluation.
茄子抗冻疮成分的网络药理学与生物评价研究。
J Agric Food Chem. 2023 Aug 2;71(30):11442-11453. doi: 10.1021/acs.jafc.3c01108. Epub 2023 Jul 19.
4
Curcuma latifolia Roscoe extract reverses inflammatory pain in mice and offers a favorable CNS safety profile.蓬莪术提取物可逆转小鼠的炎症性疼痛，并呈现出有利的中枢神经系统安全性特征。
J Ethnopharmacol. 2024 Jan 10;318(Pt A):116877. doi: 10.1016/j.jep.2023.116877. Epub 2023 Jul 12.
5
An Integrative Approach to Investigate the Mode of Action of (-)-Dendroparishiol in Bacterial Meningitis: Computer-Aided Estimation of Biological Activity and Network Pharmacology.一种综合方法研究(-)-脱氢枞醇在细菌性脑膜炎中的作用模式：基于计算机的生物活性预测和网络药理学研究。
Int J Mol Sci. 2023 Apr 29;24(9):8072. doi: 10.3390/ijms24098072.
6
Signaling pathways in rheumatoid arthritis: implications for targeted therapy.类风湿关节炎中的信号通路：靶向治疗的意义。
Signal Transduct Target Ther. 2023 Feb 17;8(1):68. doi: 10.1038/s41392-023-01331-9.
7
Roles of Oxidative Stress in Acute Tendon Injury and Degenerative Tendinopathy-A Target for Intervention.氧化应激在急性肌腱损伤和退行性肌腱病中的作用——干预靶点。
Int J Mol Sci. 2022 Mar 25;23(7):3571. doi: 10.3390/ijms23073571.
8
A Comprehensive Review on the Therapeutic Potential of Linn. in Relation to its Major Active Constituent Curcumin.关于[植物名称 Linn.]与其主要活性成分姜黄素相关治疗潜力的综合综述。 （由于原文中未明确给出植物名称，这里用[植物名称 Linn.]来表示原文中未完整写出的植物学名相关内容）
Front Pharmacol. 2022 Mar 25;13:820806. doi: 10.3389/fphar.2022.820806. eCollection 2022.
9
Guaiane-type sesquiterpenes from Curcumawenyujin.温郁金中的愈创木烷型倍半萜
Phytochemistry. 2022 Jun;198:113164. doi: 10.1016/j.phytochem.2022.113164. Epub 2022 Mar 17.
10
Anti-Inflammatory Effects of Curcumin in the Inflammatory Diseases: Status, Limitations and Countermeasures.姜黄素在炎症性疾病中的抗炎作用：现状、局限性和对策。
Drug Des Devel Ther. 2021 Nov 2;15:4503-4525. doi: 10.2147/DDDT.S327378. eCollection 2021.