Schnabl Stinna D, Klubien Jeanett, O'Rourke Colm J, Bull Nordkild Sophie, Kugler Jan-Michael, Dam Nielsen Susanne, Andersen Jesper B, Pommergaard Hans-Christian
Department of Surgery and Transplantation, Copenhagen University Hospital, Rigshospitalet, Denmark.
Hepatic Malignancy Surgical Research Unit (HEPSURU), Department of Surgery and Transplantation, Rigshospitalet, Copenhagen University Hospital, Denmark.
J Clin Exp Hepatol. 2025 Jul-Aug;15(4):102544. doi: 10.1016/j.jceh.2025.102544. Epub 2025 Mar 11.
BACKGROUND/AIMS: Several prognostic gene signatures have been proposed as predictors of the prognosis of hepatocellular carcinoma (HCC), yet none are implemented in the clinical setting. We aimed to validate two gene scores previously derived from European cohorts.
The patients who underwent liver resection for HCC at Copenhagen University Hospital, Rigshospitalet from 2014 to 2018 were included. RNA sequencing determined the expression of genes in the '' () and ' (). Univariable Cox regression assessed associations with overall and disease-free survival. These parameters were also analyzed in the The Cancer Genome Atlas Liver Hepatocellular Carcinoma (TCGA-LIHC) (n = 359) and National Institute of Health (NIH) (n = 178) cohorts.
Among 51 patients (88% male), 59% had no underlying liver disease and 25% had cirrhosis. No individual genes were significantly associated with overall survival in the Danish cohort. In the TCGA-LIHC cohort, was linked to better overall survival, and in the NIH cohort, high expression of was associated with poorer overall survival. In the TCGA-LIHC cohort, , , and were associated with poorer overall survival, while was linked to better overall survival. No genes were associated with disease-free survival.
Few individual genes significantly predicted survival in the larger cohorts, and none in the Danish cohort. However, the clinical implication of this needs further investigation.
背景/目的：已提出多种预后基因特征作为肝细胞癌（HCC）预后的预测指标，但尚无一种在临床中得到应用。我们旨在验证先前从欧洲队列中得出的两个基因评分。
纳入2014年至2018年在哥本哈根大学医院里格霍斯皮塔利接受HCC肝切除术的患者。RNA测序确定了“（）”和“（）”中基因的表达。单变量Cox回归评估与总生存期和无病生存期的关联。这些参数也在癌症基因组图谱肝细胞癌（TCGA-LIHC）队列（n = 359）和美国国立卫生研究院（NIH）队列（n = 178）中进行了分析。
在51例患者（88%为男性）中，59%无潜在肝脏疾病，25%有肝硬化。在丹麦队列中，没有单个基因与总生存期显著相关。在TCGA-LIHC队列中，“”与更好的总生存期相关，而在NIH队列中，“”的高表达与较差的总生存期相关。在TCGA-LIHC队列中，“”、“”和“”与较差的总生存期相关，而“”与更好的总生存期相关。没有基因与无病生存期相关。
在较大队列中，很少有单个基因能显著预测生存期，在丹麦队列中则没有。然而，其临床意义需要进一步研究。
