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技术说明:用于从粘性诱捕器中最佳提取和形态鉴定丽蝇(双翅目:丽蝇科)的溶剂比较

Technical Note: A comparison of solvents for optimal extraction and morphological identification of blow flies (Diptera: Calliphoridae) from sticky traps.

作者信息

Barnes Kate M, Bulling Mark T, Wosik Gosia, Twinn Alana F V, Lemon Samara, Foreman Chloe, Babiarz Kinga, Brown Katherine

机构信息

School of Science, University of Derby, Kedleston Road, DE22 1GB, UK.

School of Criminology and Criminal Justice, University of Portsmouth, Hampshire, PO1 2HY, UK.

出版信息

Forensic Sci Int Synerg. 2025 Apr 7;10:100583. doi: 10.1016/j.fsisyn.2025.100583. eCollection 2025 Jun.

DOI:10.1016/j.fsisyn.2025.100583
PMID:40248744
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12005907/
Abstract

Passive sampling techniques such as sticky traps are recommended for research studies assessing colonisation patterns of forensically important flies. However, there are no standardised protocols for the optimal removal of flies to ensure accurate morphological identification to species level. This study assessed the use of four freely available solvents (mineral oil, vegetable oil, baby oil and an orange-based solvent) in terms of facilitating extraction from sticky traps, and potential effects on subsequent identification of three blow fly species of forensic importance, and . Results indicated that species were differentially affected by the oils but, overall, the orange-based solvent had the least effect on the morphological features of each species, and therefore, was considered the best throughout the study. Additionally, the orientation of flies on the traps had no significant effect on the quality of morphological characteristics. It is recommended that the orange-based solvent method outlined in this paper is used for the removal of blow flies from sticky traps.

摘要

对于评估具有法医重要性的苍蝇定殖模式的研究,推荐使用粘性诱捕器等被动采样技术。然而,目前尚无用于从粘性诱捕器中最佳移除苍蝇以确保准确鉴定到物种水平的标准化方案。本研究评估了四种可免费获取的溶剂(矿物油、植物油、婴儿油和一种橙色基溶剂)在促进从粘性诱捕器中提取苍蝇方面的作用,以及对随后鉴定三种具有法医重要性的丽蝇物种( 、 和 )的潜在影响。结果表明,不同物种受这些油类的影响存在差异,但总体而言,橙色基溶剂对每个物种的形态特征影响最小,因此在整个研究中被认为是最佳的。此外,苍蝇在诱捕器上的朝向对形态特征的质量没有显著影响。建议使用本文所述的橙色基溶剂法从粘性诱捕器中移除丽蝇。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f249/12005907/c66fcf4332e1/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f249/12005907/00fffd0042cd/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f249/12005907/eeb257878f58/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f249/12005907/58b0ba1ddabb/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f249/12005907/40887a7ad001/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f249/12005907/c66fcf4332e1/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f249/12005907/00fffd0042cd/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f249/12005907/eeb257878f58/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f249/12005907/58b0ba1ddabb/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f249/12005907/40887a7ad001/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f249/12005907/c66fcf4332e1/gr5.jpg

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本文引用的文献

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J Econ Entomol. 2022 Jun 8;115(3):844-851. doi: 10.1093/jee/toac046.
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Small Bait Traps May Not Accurately Reflect the Composition of Necrophagous Diptera Associated to Remains.小型诱饵诱捕器可能无法准确反映与遗体相关的食尸性双翅目昆虫的组成。
Insects. 2021 Mar 20;12(3):261. doi: 10.3390/insects12030261.
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Comparison of Techniques for Sampling Adult Necrophilous Insects From Pig Carcasses.
从猪尸体上采集成年嗜尸性昆虫的技术比较
J Med Entomol. 2018 Jun 28;55(4):947-954. doi: 10.1093/jme/tjx255.
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Comparing Species Composition of Passive Trapping of Adult Flies with Larval Collections from the Body during Scene-Based Medicolegal Death Investigations.在基于现场的法医学死亡调查中,比较成年苍蝇被动诱捕与尸体幼虫采集的物种组成。
Insects. 2017 Mar 24;8(2):36. doi: 10.3390/insects8020036.
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