Gao Yuxuan, Guo Weiqing, Wei Qianhui, Wang Rongyue, Liu Yanan, Han Fang, Wei Feng
State Key Laboratory of Advanced Materials for Intelligent Sensing, GRINM Group Co., Ltd., Beijing, 100088, China; GRINM (Guangdong) Institute for Advanced Materials and Technology, Foshan, 528000, China; General Research Institute for Nonferrous Metals, Beijing, 100088, China.
State Key Laboratory of Advanced Materials for Intelligent Sensing, GRINM Group Co., Ltd., Beijing, 100088, China; GRINM (Guangdong) Institute for Advanced Materials and Technology, Foshan, 528000, China.
Talanta. 2025 Oct 1;293:128148. doi: 10.1016/j.talanta.2025.128148. Epub 2025 Apr 15.
Formaldehyde (HCHO), as a representative biomarker associated with lung cancer screening, plays a key role in distinguishing healthy individuals from those with diseases through its ppb-level concentration differences. Therefore, achieving high sensitivity and low limit of detection (LOD) for specific detection of HCHO is critically important. In this work, a HCHO electrochemical sensor was prepared using a two-step drop-coating method on a flexible planar screen-printed electrode, with 60 % Pt/C-Nafion catalyst as the sensing layer and Nafion as the solid-state electrolyte. At a working potential of 0.5 V, high sensitivity (0.197 μA/ppm), a wide linear response range (HCHO concentration from 500 ppb to 100 ppm) and a low LOD (2.5 ppb) have been achieved for HCHO detection at room temperature. The sensor also exhibits excellent selectivity, consistency and long-term stability (>15 days). Through ultra-low concentration sensing tests of 50-400 ppb HCHO using a fluorine membrane gas bag, the sensor demonstrates good linearity with a sensitivity of 0.8 nA/ppb, which can effectively distinguish between exhaled breath from healthy individuals and simulated lung cancer patients. The appropriate selection of working potential, efficient construction of three-phase boundary (TPB) and excellent electrocatalytic performance of the Pt/C catalyst enable the realization of high-performance HCHO detection. Additionally, the sensor preparation method is simple and cost-effective. Therefore, the proposed Nafion-based HCHO electrochemical sensor is expected to provide a promising sensing platform for lung cancer screening in clinical diagnosis and indoor HCHO concentration monitoring.
甲醛（HCHO）作为与肺癌筛查相关的代表性生物标志物，通过其在十亿分比水平的浓度差异，在区分健康个体和患病个体方面发挥着关键作用。因此，实现对HCHO的特异性检测的高灵敏度和低检测限（LOD）至关重要。在这项工作中，采用两步滴涂法在柔性平面丝网印刷电极上制备了一种HCHO电化学传感器，以60%的Pt/C-全氟磺酸催化剂作为传感层，全氟磺酸作为固态电解质。在0.5 V的工作电位下，该传感器在室温下对HCHO检测实现了高灵敏度（0.197 μA/ppm）、宽线性响应范围（HCHO浓度从500 ppb到100 ppm）和低LOD（2.5 ppb）。该传感器还表现出优异的选择性、一致性和长期稳定性（>15天）。通过使用氟膜气袋对50 - 400 ppb HCHO进行超低浓度传感测试，该传感器显示出良好的线性，灵敏度为0.8 nA/ppb，能够有效区分健康个体和模拟肺癌患者的呼出气体。工作电位的适当选择、三相边界（TPB）的有效构建以及Pt/C催化剂的优异电催化性能使得高性能的HCHO检测得以实现。此外，该传感器制备方法简单且具有成本效益。因此，所提出的基于全氟磺酸的HCHO电化学传感器有望为临床诊断中的肺癌筛查和室内HCHO浓度监测提供一个有前景的传感平台。
