Chen Yanfang, Zhang Zhe, Li Ling, Zhang Mingzhi, Li Zhaoming
Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
J Transl Med. 2025 Apr 19;23(1):459. doi: 10.1186/s12967-025-06456-9.
Castleman Disease (CD) is a rare lymphoproliferative disorder of uncertain etiology, primarily characterized by painless lymphadenopathy. To further understand the possible pathogenesis of the disease, we utilized spatial transcriptomics to explore CD.
Spatial transcriptomics was performed on FFPE samples from lymph nodes of three CD patients with different histologic types and one normal lymph node.
The sample was categorized by analysis into 14 clusters, including B cells, plasma cells, BECs, LECs, CD4 + T cells, CD8 + T cells, macrophages, monocytes, cycling cells, myofibroblasts, mDCs, NKCs, Tfh and NESCs. Our study showed that the lesion cells of CD might be B cells. In addition to this, we found that mesenchymal cells, especially myofibroblasts, play an important role in disease progression and there was a large heterogeneity of cellular communication between cell clusters in different histologic types of CD.
Through this study we have gained a better understanding of the pathogenesis of CD. It provides new ideas for the subsequent exploration of CD and directions for the development of new clinical drugs.
卡斯特曼病（CD）是一种病因不明的罕见淋巴增殖性疾病，主要特征为无痛性淋巴结病。为进一步了解该疾病的可能发病机制，我们利用空间转录组学技术对CD进行了探索。
对来自三名不同组织学类型的CD患者淋巴结的福尔马林固定石蜡包埋（FFPE）样本以及一个正常淋巴结进行了空间转录组学分析。
通过分析将样本分为14个簇，包括B细胞、浆细胞、BECs、LECs、CD4 + T细胞、CD8 + T细胞、巨噬细胞、单核细胞、循环细胞、肌成纤维细胞、mDCs、NKCs、Tfh和NESCs。我们的研究表明，CD的病变细胞可能是B细胞。除此之外，我们发现间充质细胞，尤其是肌成纤维细胞，在疾病进展中起重要作用，并且不同组织学类型的CD细胞簇之间的细胞通讯存在很大异质性。
通过本研究，我们对CD的发病机制有了更好的理解。它为后续CD的探索提供了新思路，也为新临床药物的开发指明了方向。
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