Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Harvard Stem Cell and Regenerative Biology, Cambridge, MA, USA.
Nat Biotechnol. 2023 Jun;41(6):773-782. doi: 10.1038/s41587-022-01448-2. Epub 2022 Oct 3.
The formation and maintenance of tissue integrity requires complex, coordinated activities by thousands of genes and their encoded products. Until recently, transcript levels could only be quantified for a few genes in tissues, but advances in DNA sequencing, oligonucleotide synthesis and fluorescence microscopy have enabled the invention of a suite of spatial transcriptomics technologies capable of measuring the expression of many, or all, genes in situ. These technologies have evolved rapidly in sensitivity, multiplexing and throughput. As such, they have enabled the determination of the cell-type architecture of tissues, the querying of cell-cell interactions and the monitoring of molecular interactions between tissue components. The rapidly evolving spatial genomics landscape will enable generalized high-throughput genomic measurements and perturbations to be performed in the context of tissues. These advances will empower hypothesis generation and biological discovery and bridge the worlds of tissue biology and genomics.
组织完整性的形成和维持需要数千个基因及其编码产物的复杂协调活动。直到最近，只能对组织中少数几个基因的转录水平进行定量，但是 DNA 测序、寡核苷酸合成和荧光显微镜的进步使得发明了一整套空间转录组学技术成为可能，这些技术能够原位测量许多或所有基因的表达。这些技术在灵敏度、多重性和通量方面迅速发展。因此，它们使组织中细胞类型结构的确定、细胞间相互作用的查询以及组织成分之间分子相互作用的监测成为可能。快速发展的空间基因组学领域将使在组织背景下进行高通量基因组测量和干扰成为可能。这些进展将为假设生成和生物发现提供动力，并弥合组织生物学和基因组学之间的鸿沟。
