Górniak Dorota, Świątecki Aleksander, Kowalik Jakub, Grzesiak Jakub, Jastrzębski Jan, Zdanowski Marek K
Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1a, Olsztyn, 10-719, Poland.
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5A, Warszawa, 02- 106, Poland.
Sci Rep. 2025 Apr 19;15(1):13615. doi: 10.1038/s41598-025-97205-x.
This is the first study to demonstrate a relationship between antagonistic interactions with antibiotic resistance within flavobacterial strains, a component of polar-region microbial mats. These strains were derived from ephemeral freshwater reservoirs, i.e. ponds and streams of the periglacial zone of Ecology Glacier (King George Island, maritime Antarctica). The study demonstrated the strains' surprisingly high phylogenetic diversity, with 20 species among 50 isolates. Flavobacteria were characterised by different patterns of antagonism and sensitivity to antimicrobials. 29 strains produced substances inhibiting the growth of other isolates, with 21 strains being sensitive to such compounds; 34 strains were multidrug-resistant (MDR). The antibiotic resistance index (ARI) demonstrated a significantly higher proportion of MDR strains and ARI ≥ 0.2 in stream mats (87%) as compared to the strains derived from pond mats (55%). A strong correlation was observed between the strains' antagonistic potential and antibiotic resistance. An important role in these phenomena is accomplished by the "super bacteria" strains that effectively accumulate numerous traits associated with antagonistic potential and can be involved in the potential transfer of these traits. The results of the study demonstrate that there are individual patterns of antagonistic interactions and antibiotic resistance among the biotic components of mats.
这是第一项证明黄杆菌菌株（极地地区微生物垫的一个组成部分）内拮抗相互作用与抗生素抗性之间存在关联的研究。这些菌株源自短暂性淡水水库，即生态冰川（南极海洋性乔治王岛）冰缘区的池塘和溪流。该研究证明了这些菌株具有惊人的高系统发育多样性，在50个分离株中有20个物种。黄杆菌的特征在于不同的拮抗模式和对抗菌剂的敏感性。29个菌株产生抑制其他分离株生长的物质，其中21个菌株对这类化合物敏感；34个菌株具有多重耐药性（MDR）。抗生素抗性指数（ARI）表明，与源自池塘微生物垫的菌株（55%）相比，溪流微生物垫中多重耐药菌株的比例和ARI≥0.2的比例显著更高（87%）。观察到菌株的拮抗潜力与抗生素抗性之间存在很强的相关性。“超级细菌”菌株在这些现象中发挥着重要作用，它们能有效积累与拮抗潜力相关的众多特性，并可能参与这些特性的潜在转移。研究结果表明，微生物垫的生物成分之间存在个体性的拮抗相互作用和抗生素抗性模式。
