Akulava Volha, Miamin Uladzislau, Akhremchuk Katsiaryna, Valentovich Leonid, Dolgikh Andrey, Shapaval Volha
Faculty of Science and Technology, Norwegian University of Life Sciences, 1432 Ås, Norway.
Faculty of Biology, Belarusian State University, 220030 Minsk, Belarus.
Biology (Basel). 2022 Jul 29;11(8):1143. doi: 10.3390/biology11081143.
In this study, for the first time, we report the identification and characterization of culturable fast-growing bacteria isolated from the sea-affected temporary meltwater ponds (MPs) in the East Antarctica area of the Vecherny region (-67.656317, 46.175058) of the Thala Hills Oasis, Enderby Land. Water samples from the studied MPs showed alkaline pH (from 8.0 to 10.1) and highly varied total dissolved solids (86-94,000 mg/L). In total, twenty-nine bacterial isolates were retrieved from the studied MPs. The phylogenetic analysis based on 16S rRNA gene sequence similarities showed that the isolated bacteria belong to the phyla Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes and the twelve genera , , , , , , , , , , , and . Most of the isolated bacteria were psychrotrophs and showed the production of one or more extracellular enzymes. Lipolytic and proteolytic activities were more prevalent among the isolates. Five isolates from the Actinobacteria phylum and one isolate from the Bacteroidetes phylum had strong pigmentation. Antibiotic susceptibility testing revealed that most of the isolates are resistant to at least one antibiotic, and seven isolates showed multi-resistance.
在本研究中，我们首次报告了从恩德比地塔拉山绿洲韦尔尼地区（-67.656317, 46.175058）东南极地区受海洋影响的临时融水池塘（MPs）中分离出的可培养快速生长细菌的鉴定和特征。来自所研究MPs的水样显示pH呈碱性（8.0至10.1），总溶解固体含量变化很大（86 - 94,000 mg/L）。总共从所研究的MPs中获得了29株细菌分离株。基于16S rRNA基因序列相似性的系统发育分析表明，分离出的细菌属于变形菌门、放线菌门、厚壁菌门和拟杆菌门，以及12个属，分别为 、 、 、 、 、 、 、 、 、 、 、 和 。大多数分离出的细菌是嗜冷菌，并显示出产生一种或多种细胞外酶。脂肪分解和蛋白水解活性在分离株中更为普遍。来自放线菌门的5个分离株和来自拟杆菌门的1个分离株具有强烈的色素沉着。抗生素敏感性测试表明，大多数分离株对至少一种抗生素耐药，7个分离株显示多重耐药。
