Merino Lina E, Llana Mariángeles Noto, Garófalo Ailín, Trejo Fernando M, Golowczyc Marina A
Universidad Nacional de Hurlingham, Av. Gdor. Vergara 2222, Hurlingham, Buenos Aires, B1688GEZ, Argentina.
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CICPBA), Argentina.
Curr Res Microb Sci. 2025 Apr 2;8:100384. doi: 10.1016/j.crmicr.2025.100384. eCollection 2025.
(SE) is a pathogenic bacterium commonly found on the surface of eggshells. In this study, we investigated the biofilm formation of a specific strain of , CIDCA 115 (SE 115) on eggshells. Additionally, we examined the impact of strains 83113 and 8321, as well as 83114, and their metabolites present in the supernatant on the biofilm formation of SE 115. Scanning electron microscopy revealed that SE 115 formed a mature biofilm structure on the eggshell. During co-incubation and pre-incubation, lactic acid bacteria strains significantly reduced the formation of SE 115 biofilm ( < 0.05 and < 0.01, respectively) compared to SE 115 grown alone. The cell-free supernatants of lactic acid bacteria also exhibited a reduction in SE 115 biofilm formation and modified its structure. Co-incubation with SE 115 induced alterations in the composition of biofilm matrix components, notably in the levels of fimbria curli and cellulose. The qPCR analysis revealed that, after 48 h of incubation, the expression of the gene, a critical regulator of biofilm formation, remained unchanged compared to planktonic cells. However, genes associated with the production of biofilm matrix components, curli () and cellulose (), exhibited heightened expression in the presence of lactic acid bacteria compared to the planktonic state. This study highlights the potential of lactic acid bacteria strains and their metabolites as innovative strategies for managing biofilm formation in the context of poultry production.
肠炎沙门氏菌（SE）是一种常见于蛋壳表面的病原菌。在本研究中，我们调查了特定肠炎沙门氏菌菌株CIDCA 115（SE 115）在蛋壳上的生物膜形成情况。此外，我们还研究了83113和8321菌株以及83114菌株及其存在于上清液中的代谢产物对SE 115生物膜形成的影响。扫描电子显微镜显示，SE 115在蛋壳上形成了成熟的生物膜结构。在共孵育和预孵育过程中，与单独生长的SE 115相比，乳酸菌菌株显著减少了SE 115生物膜的形成（分别为P<0.05和P<0.01）。乳酸菌的无细胞上清液也使SE 115生物膜形成减少并改变了其结构。与SE 115共孵育诱导了生物膜基质成分组成的改变，特别是菌毛卷曲蛋白和纤维素的水平。定量聚合酶链反应（qPCR）分析显示，孵育48小时后，生物膜形成关键调节基因的表达与浮游细胞相比没有变化。然而，与生物膜基质成分卷曲蛋白（csgA）和纤维素（bcsA）产生相关的基因在有乳酸菌存在时与浮游状态相比表现出更高的表达。本研究强调了乳酸菌菌株及其代谢产物作为家禽生产中管理肠炎沙门氏菌生物膜形成的创新策略的潜力。
