Yuan Hui, Sun Daofan, Lu Bo, Meng Bo, Liu Rongjun, Wang Ruichun, Xing Xiuzhong, Ji Yiqin, Ming Qianyu, Wang Qiusheng, Chen Junping
Department of Anesthesiology, Ningbo No. 2 Hospital, Ningbo 315010, China.
Department of Pain, Ningbo No. 2 Hospital, Ningbo 315010, China.
IBRO Neurosci Rep. 2025 Apr 3;18:545-553. doi: 10.1016/j.ibneur.2025.03.010. eCollection 2025 Jun.
Many studies have shown that postoperative pain aggravates perioperative neurocognitive disorder (PND). In this study we aimed to clarify the effect of preoperative inflammatory pain on postoperative pain and cognitive function.
We established the inflammatory pain model by injected complete freund adjuvant (CFA) and the PND model by tibial fracture surgery in 14- month-old C57BL/6 mice. The paw withdrawal threshold and body weight of the mice were measured 7 days before surgery and 3 days after surgery. On the third postoperative day, mice were subjected to behavioral testing or sacrificed to collect brain tissue.
The result shows that CFA exacerbated postoperative pain and cognitive dysfunction in mice, enhanced surgery-induced activation of microglia and astrocytes in the hippocampus, and increased surgery-induced the overexpression of IL-1β, IL-6, and TNF-α, as well as aggravated the decreased expression of α7nAChR and the overexpression of HMGB1 in the hippocampus induced by surgery.
Our study shows that preoperative inflammatory pain further aggravates postoperative pain and neurocognitive dysfunction in aged rats, and the mechanism may be related to neuroinflammation caused by α7nAChR-mediated CAP dysfunction and high release of HMGB1.
许多研究表明，术后疼痛会加重围手术期神经认知障碍（PND）。在本研究中，我们旨在阐明术前炎性疼痛对术后疼痛和认知功能的影响。
我们通过向14月龄的C57BL/6小鼠注射完全弗氏佐剂（CFA）建立炎性疼痛模型，并通过胫骨骨折手术建立PND模型。在手术前7天和手术后3天测量小鼠的爪部退缩阈值和体重。在术后第三天，对小鼠进行行为测试或处死以收集脑组织。
结果显示，CFA加剧了小鼠的术后疼痛和认知功能障碍，增强了手术诱导的海马小胶质细胞和星形胶质细胞的激活，并增加了手术诱导的白细胞介素-1β、白细胞介素-6和肿瘤坏死因子-α的过度表达，以及加重了手术诱导的海马中α7烟碱型乙酰胆碱受体表达降低和高迁移率族蛋白B1的过度表达。
我们的研究表明，术前炎性疼痛会进一步加重老年大鼠的术后疼痛和神经认知功能障碍，其机制可能与α7烟碱型乙酰胆碱受体介导的胆碱能功能障碍和高迁移率族蛋白B1的高释放引起的神经炎症有关。
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