Uğran R, Koral Taşçı S
Department of Veterinary Medicine, Vocational School of Technical Sciences. Batman University, Batman, Turkey.
Department of Histology and Embryology, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkey.
Arch Razi Inst. 2024 Aug 1;79(4):815-826. doi: 10.32592/ARI.2024.79.4.815. eCollection 2024 Aug.
The study was designed to investigate the effect of curcumin, known for its antidiabetic properties, on the immunohistochemical localization and gene expression of glucagon-like peptide-1 receptor (GLP-1R) in the liver tissues of experimental diabetic rats using reverse transcription polymerase chain reaction (RT-PCR). For this, 24 Sprague-Dawley rats were divided into four groups-control, sham, diabetic, and diabetic + curcumin groups. The control group received no treatment, and 50 mg/kg streptozotocin was administered to the rats in the diabetic and diabetic + curcumin groups received 50 mg/kg streptozotocin. Once diabetes had been established, 100 mg/kg of curcumin was administered intraperitoneally to rats in the diabetic + curcumin group for a period of 21 days. Thesham group was administeredintraperitoneal ethanol and isotonic sodium chloride solution. At the ends of the experiment,tissues were subjected to histological and immunohistochemical examination to ascertain the localization of GLP-1R. Additionally, RT-PCR was employed to determine the levels of GLP-1R gene expression.The histological examinations revealed that the tissue samples from the control and sham groups exhibited a normal histological structure. In contrasr, the diabetic group displayed a range of degenerative changes, including enlargement of the sinusoidal wall enlargement and vacuolization of the hepatocytes. Furthermore, these degenerative findings were mitigated in the diabetic + curcumin group. In the immunohistochemical examinations, the majority of hepatocytes surrounding the vena centralis, as well as some endothelial, and some Kupffer cells,exhibited positively for GLP-1R. The diabetic group exhibited reduced immunoreactivity, while the diabetic + curcumin group demonstrated elevated immunoreactivity compared to the diabetes group. With regard to the molecular analysis, the mean expression level was observed to be higher in the diabetes + curcumin group. However, no significant difference in GLP-1R gene expression was identified between the groups. In conclusion, the administration of curcumin was observed to enhance GLP-1R expression in the liver of the rats with diabetes. Given that GLP-1R is a targets for diabetes treatment, curcumin can be used as a viable therapeutic agent for treating diabetes and alleviating its complications.
本研究旨在利用逆转录聚合酶链反应（RT-PCR），研究以其抗糖尿病特性而闻名的姜黄素对实验性糖尿病大鼠肝脏组织中胰高血糖素样肽-1受体（GLP-1R）的免疫组织化学定位和基因表达的影响。为此，将24只Sprague-Dawley大鼠分为四组——对照组、假手术组、糖尿病组和糖尿病+姜黄素组。对照组未接受治疗，糖尿病组和糖尿病+姜黄素组的大鼠接受50mg/kg链脲佐菌素注射。一旦糖尿病确诊，糖尿病+姜黄素组的大鼠腹腔注射100mg/kg姜黄素，持续21天。假手术组腹腔注射乙醇和等渗氯化钠溶液。在实验结束时，对组织进行组织学和免疫组织化学检查，以确定GLP-1R的定位。此外，采用RT-PCR测定GLP-1R基因表达水平。组织学检查显示，对照组和假手术组的组织样本呈现正常的组织结构。相比之下，糖尿病组出现了一系列退行性变化，包括肝血窦壁增厚和肝细胞空泡化。此外，糖尿病+姜黄素组的这些退行性表现有所减轻。在免疫组织化学检查中，中央静脉周围的大多数肝细胞以及一些内皮细胞和枯否细胞对GLP-1R呈阳性反应。糖尿病组的免疫反应性降低，而糖尿病+姜黄素组与糖尿病组相比，免疫反应性升高。关于分子分析，糖尿病+姜黄素组的平均表达水平较高。然而，各组之间GLP-1R基因表达没有显著差异。总之，观察到姜黄素给药可增强糖尿病大鼠肝脏中GLP-1R的表达。鉴于GLP-1R是糖尿病治疗的靶点，姜黄素可作为治疗糖尿病及其并发症的可行治疗药物。
