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通过RNA测序和生物信息学分析子宫内膜异位症中的环状RNA以获取表达谱。

Circular RNAs in endometriosis analyzed through RNA sequencing and bioinformatics for expression profile.

作者信息

Yue Yan, Qian Jing, Gui Dandan, Lu Bin

机构信息

Department of Gynecology, The First People's Hospital of Wuhu, Wuhu, China.

出版信息

PeerJ. 2025 Apr 16;13:e19298. doi: 10.7717/peerj.19298. eCollection 2025.

DOI:10.7717/peerj.19298
PMID:40256743
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12009022/
Abstract

BACKGROUND

Endometriosis severely affects women's physical and mental health; it is particularly important to find targets for the treatment and diagnosis of endometriosis.

METHOD

This research aimed to investigate the circRNA expression pattern in endometriosis, a type of non-coding RNA that can modulate parental gene expression by acting as miRNA sponges. Through high-throughput sequencing, we analyzed the circRNA expression profile in endometriosis patients in comparison to individuals without the condition.

RESULTS

We detected 371 circular RNAs (circRNAs) showing increased expression and 308 circRNAs displaying decreased expression levels. To validate these findings, we employed quantitative real-time PCR (qRT-PCR) to confirm the expression of the top three differential expressed circRNAs listed in circBase. We inferred potential roles of these differentially expressed circRNAs in endometriosis development by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Moreover, by examining the circRNA-microRNA-target gene network, we uncovered a plausible mechanism. Specifically, interactions involving the markedly upregulated hsa_circ_000005 and significantly downregulated hsa_circ_000011 with miR-5787 may influence downstream targets, potentially contributing to the pathogenesis of endometriosis. Our study offers a foundational and crucial circRNA expression profile within the framework of endometriosis.

摘要

背景

子宫内膜异位症严重影响女性身心健康；寻找子宫内膜异位症的治疗和诊断靶点尤为重要。

方法

本研究旨在调查子宫内膜异位症中的环状RNA（circRNA）表达模式，circRNA是一种非编码RNA，可通过充当微小RNA（miRNA）海绵来调节亲本基因表达。通过高通量测序，我们分析了子宫内膜异位症患者与未患该病个体的circRNA表达谱。

结果

我们检测到371个环状RNA（circRNA）表达增加，308个circRNA表达水平降低。为验证这些发现，我们采用定量实时聚合酶链反应（qRT-PCR）来确认circBase中列出的前三个差异表达circRNA的表达。我们通过基因本体论（GO）和京都基因与基因组百科全书（KEGG）富集分析推断这些差异表达circRNA在子宫内膜异位症发展中的潜在作用。此外，通过研究circRNA-微小RNA-靶基因网络，我们发现了一种可能的机制。具体而言，显著上调的hsa_circ_000005和显著下调的hsa_circ_000011与miR-5787之间的相互作用可能影响下游靶点，这可能是子宫内膜异位症发病机制的原因。我们的研究在子宫内膜异位症框架内提供了一个基础且关键的circRNA表达谱。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b20/12009022/503e5c1f4581/peerj-13-19298-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b20/12009022/a38585a11bf8/peerj-13-19298-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b20/12009022/faa1dd313efd/peerj-13-19298-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b20/12009022/9fa22f3155de/peerj-13-19298-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b20/12009022/630b45e7630a/peerj-13-19298-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b20/12009022/503e5c1f4581/peerj-13-19298-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b20/12009022/a38585a11bf8/peerj-13-19298-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b20/12009022/faa1dd313efd/peerj-13-19298-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b20/12009022/9fa22f3155de/peerj-13-19298-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b20/12009022/630b45e7630a/peerj-13-19298-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b20/12009022/503e5c1f4581/peerj-13-19298-g005.jpg

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本文引用的文献

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Gynecol Minim Invasive Ther. 2024 Feb 23;13(1):62-63. doi: 10.4103/gmit.gmit_52_23. eCollection 2024 Jan-Mar.
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Discordance in Histopathological versus Clinical Diagnosis of a Paracolpium Endometrioma - A Diagnostic Challenge.阴道旁子宫内膜异位囊肿组织病理学诊断与临床诊断的不一致性——一项诊断挑战
Gynecol Minim Invasive Ther. 2024 Jan 12;13(1):64-65. doi: 10.4103/gmit.gmit_48_22. eCollection 2024 Jan-Mar.
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Role of Cyclins and Cytoskeletal Proteins in Endometriosis: Insights into Pathophysiology.
细胞周期蛋白和细胞骨架蛋白在子宫内膜异位症中的作用：对病理生理学的见解
Cancers (Basel). 2024 Feb 19;16(4):836. doi: 10.3390/cancers16040836.
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Clinical Characteristics and Local Histopathological Modulators of Endometriosis and Its Progression.子宫内膜异位症及其进展的临床特征和局部组织病理学调节因子。
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