High-performance liquid chromatographic analysis of citric acid, inositol and fructose in boar and bull seminal plasma.

The efficiency of ultrafiltration and high-performance liquid chromatography (HPLC) in the routine analysis of dialysable sugar alcohols, organic acids and carbohydrates in boar and bull seminal plasma is evaluated. Permitting a simple and rapid separation of protein-free samples, ultrafiltration reduces the time of HPLC analysis by 40 minutes as compared to protein precipitation by means of methanol. Under optimal flow-conditions the separation of citric acid, inositol and fructose is achieved in 16 minutes. Moreover, the use of silicon-oil for separating seminal plasma within seconds is described.