Oefner P, Bonn G, Bartsch G
Andrologia. 1985 May-Jun;17(3):250-3. doi: 10.1111/j.1439-0272.1985.tb00997.x.
The efficiency of ultrafiltration and high-performance liquid chromatography (HPLC) in the routine analysis of dialysable sugar alcohols, organic acids and carbohydrates in boar and bull seminal plasma is evaluated. Permitting a simple and rapid separation of protein-free samples, ultrafiltration reduces the time of HPLC analysis by 40 minutes as compared to protein precipitation by means of methanol. Under optimal flow-conditions the separation of citric acid, inositol and fructose is achieved in 16 minutes. Moreover, the use of silicon-oil for separating seminal plasma within seconds is described.
评估了超滤和高效液相色谱法（HPLC）在公猪和公牛精液中可透析糖醇、有机酸和碳水化合物常规分析中的效率。超滤可实现无蛋白样品的简单快速分离，与用甲醇沉淀蛋白质相比，可将HPLC分析时间缩短40分钟。在最佳流动条件下，16分钟内可实现柠檬酸、肌醇和果糖的分离。此外，还介绍了使用硅油在几秒钟内分离精液的方法。
