Kostiuk P G, Kryshtal' O A, Tsyndrenko A Ia
Neirofiziologiia. 1977;9(1):69-77.
A newly developed method of internal dialysis was applied together with the voltage clamp method to the isolated neurons of molluscs Helix pomatia and Limnea stagnalis. Effect of Ca on the Ca inward and K delayed outward currents was investigated. Removal of Ca from the external medium was equivalent to 25-35 mV hyperpolarization of the membrane. Internal Ca in concentrations up to 3.5-10-(7)M caused insignificant depression of K outward current. 5.7-10-(8)M of internal Ca completely blocked Ca inward current. Ca-chelating agents applied either internally or externally caused an appearance of Na inward current transferred through the Ca channels.
一种新开发的内部透析方法与电压钳法一起应用于软体动物罗马蜗牛和静水椎实螺的离体神经元。研究了钙对钙内向电流和钾延迟外向电流的影响。从外部介质中去除钙相当于使膜超极化25 - 35毫伏。浓度高达3.5×10⁻⁷摩尔/升的内部钙对钾外向电流的抑制作用不明显。5.7×10⁻⁸摩尔/升的内部钙完全阻断了钙内向电流。无论是在内部还是外部施加钙螯合剂都会导致通过钙通道转移的钠内向电流的出现。
