Peng Jianhua, Wu Yue, Pang Jinwei, Sun Xiaochuan, Chen Ligang, Chen Yue, Tang Jiping, Zhang John H, Jiang Yong
a Department of Neurosurgery , the Affiliated Hospital of Southwest Medical University , Luzhou , China.
b Department of Physiology and Pharmacology , School of Medicine, Loma Linda University , Loma Linda , CA , USA.
Brain Inj. 2019;33(6):701-711. doi: 10.1080/02699052.2018.1531310. Epub 2018 Oct 8.
The endovascular filament-perforation model turned out to become the most popular one for the reproduction of prominent pathophysiological features observed after human subarachnoid haemorrhage (SAH). However, few studies have considered methods that may minimize surgically induced injury. This study described an improved and simplified surgical procedure in which a single clip is placed at the external carotid artery (ECA). Male C57BL/6 mice were given either a classic endovascular filament SAH model, improved endovascular filament SAH model or sham injury. Multiple strategies, including MRI with T-weighted imaging and F-FDG PET/CT scanning, were performed to compare the improved and classic SAH models. The new method of filament model resulted a typical pathophysiological progress of early brain injury (EBI), including cerebral oedema, blood brain barrier (BBB) disruption, neuronal apoptosis and microglia activation. The improved SAH model is characterized by a shorter operation time (15.65 ± 0.64 min vs. 21.75 ± 0.94 min), reduced surgically induced injury (decreased F-FDG standardized uptake values (SUV): 1.7 ± 0.07 vs. 2.02 ± 0.11), and stable cerebral perfusion before SAH. The improved surgical technique appears to be a feasible tool for experimental and translational studies of SAH.
血管内丝线穿刺模型已成为模拟人类蛛网膜下腔出血（SAH）后显著病理生理特征的最常用模型。然而，很少有研究考虑可将手术诱导损伤降至最低的方法。本研究描述了一种改进且简化的手术方法，即在外颈动脉（ECA）处放置单个夹子。将雄性C57BL/6小鼠分为经典血管内丝线SAH模型组、改良血管内丝线SAH模型组或假手术损伤组。采用多种策略，包括T加权成像的MRI和F-FDG PET/CT扫描，对改良和经典SAH模型进行比较。丝线模型的新方法导致了早期脑损伤（EBI）典型的病理生理进程，包括脑水肿、血脑屏障（BBB）破坏、神经元凋亡和小胶质细胞激活。改良的SAH模型具有手术时间更短（15.65±0.64分钟对21.75±0.94分钟）、手术诱导损伤减少（F-FDG标准化摄取值（SUV）降低：1.7±0.07对2.02±0.11）以及SAH前脑灌注稳定的特点。改良的手术技术似乎是SAH实验和转化研究的一种可行工具。
