De Nollin S, Borgers M, Jacob W, Wellens D
Angiology. 1985 May;36(5):297-304. doi: 10.1177/000331978503600506.
In vitro exposure of depolarized caudal artery preparations of the rat to a high calcium concentration resulted in a strong contraction of the smooth muscle cells. This muscle contraction was suppressed by flunarizine. It was shown cytochemically, using the combined oxalate-pyroantimonate method for the localization of calcium, that a considerable amount of electron-dense precipitate was seen over the depolarized muscle cells after incubation in a calcium containing medium. On the other hand this precipitate was not present on the smooth muscle cells when flunarizine was added to this incubation medium. The reaction product was only present in the extracellular space. These results were controlled by Laser Microprobe Mass Analysis. By evaporating and ionizing small parts of the smooth muscle cells (+/- 1 micron), it was confirmed that the cytochemical method indeed demonstrated calcium, with negligible interference of other cations.
将大鼠去极化尾动脉制剂在体外暴露于高钙浓度下会导致平滑肌细胞强烈收缩。这种肌肉收缩被氟桂利嗪抑制。使用草酸盐 - 焦锑酸盐联合方法进行钙定位的细胞化学研究表明，在含钙培养基中孵育后，去极化的肌肉细胞上可见大量电子致密沉淀物。另一方面，当向该孵育培养基中加入氟桂利嗪时，平滑肌细胞上不存在这种沉淀物。反应产物仅存在于细胞外空间。这些结果通过激光微探针质谱分析得到验证。通过蒸发和电离平滑肌细胞的小部分（±1微米），证实细胞化学方法确实显示了钙，其他阳离子的干扰可忽略不计。
