Deng Jianxiong, Zhang Yihui, Xie Weiqi, Li Liang, Li Qiang
Spine Surgery Department of the Second People's Hospital of Hunan Province, Changsha, People's Republic of China.
Hunan University of Traditional Chinese Medicine, Changsha, People's Republic of China.
J Spinal Cord Med. 2025 Apr 22:1-12. doi: 10.1080/10790268.2025.2460303.
To explore the effects of Buyang Huanwu Decoction (BYHWD) on neurons in the red nucleus of rats with spinal cord injury (SCI) based on autophagy.
120 Sprague-Dawley (SD) rats were randomly divided into 6 groups: Control Group, SCI Group, Bafilomycin A1 Group, Rapamycin Group, BYHWD low-dose group (BL Group, 6.25 g/kg), BYHWD high-dose group (BH Group, 25.00 g/kg), with 20 animals in each group. A rat rubrospinal tract (RST) transection model was established and treated for 28 days. The recovery of motor function of rats was observed through inclined plate test and spontaneous upright exploratory behavior test. Nissl's staining was used to observe the cell morphology of injured red nucleus neurons. Reverse Transcription PCR (RT-PCR) and immunofluorescence were used to detect the expression of ATG5 and Beclin1 mRNA. The Western blot method was used to observe the expression levels of Synaptophysin (SYP), Synaptosomal-associated Protein of 25 kDa (SNAP-25), Postsynaptic density protein 95 (PSD-95), ATG5, and Beclin1 proteins in red nucleus tissue.
Compared with the SCI group, both BL and BH groups significantly improved the forelimb motor function and improved the status of red nucleus neurons in SCI rats. BYHWD increased SYP, SNAP-25, PSD-95, and decreased the red core Beclin1 and ATG5.
BYHWD enhances synaptic regeneration and limb activity in red nucleus neurons of SCI rats by inhibiting autophagy.
基于自噬探讨补阳还五汤（BYHWD）对脊髓损伤（SCI）大鼠红核神经元的影响。
将120只Sprague-Dawley（SD）大鼠随机分为6组：对照组、SCI组、巴弗洛霉素A1组、雷帕霉素组、补阳还五汤低剂量组（BL组，6.25 g/kg）、补阳还五汤高剂量组（BH组，25.00 g/kg），每组20只。建立大鼠红核脊髓束（RST）横断模型并治疗28天。通过斜板试验和自发直立探索行为试验观察大鼠运动功能的恢复情况。采用尼氏染色观察损伤红核神经元的细胞形态。采用逆转录聚合酶链反应（RT-PCR）和免疫荧光检测ATG5和Beclin1 mRNA的表达。采用蛋白质免疫印迹法观察红核组织中突触素（SYP）、25 kDa突触体相关蛋白（SNAP-25）、突触后致密蛋白95（PSD-95）、ATG5和Beclin1蛋白的表达水平。
与SCI组相比，BL组和BH组均显著改善了SCI大鼠的前肢运动功能，改善了红核神经元的状态。补阳还五汤增加了SYP、SNAP-25、PSD-95的表达，并降低了红核中Beclin1和ATG5的表达。
补阳还五汤通过抑制自噬增强SCI大鼠红核神经元的突触再生和肢体活动。
