Babalhakam Hossein Khoori, Maleki Mohsen, Noorbakhsh Mohammad Foad, Safari Nikroo Khosro, Hayati Farzad
Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Vet Med Sci. 2025 May;11(3):e70345. doi: 10.1002/vms3.70345.
Burn injuries are a significant clinical challenge due to excessive oxidative stress, inflammation and progressive tissue necrosis, leading to delayed healing and functional impairment. Ethyl pyruvate (EP) has been shown to possess potent anti-inflammatory, antioxidant, anti-apoptotic and anti-fibrotic properties, making it a promising therapeutic agent for burn wound treatment. This study aimed to evaluate the efficacy of EP in promoting burn wound healing and preventing tissue necrosis.
Following the induction of thermal damage, 30 male Wistar rats were randomly allocated to one of three distinct groups. The Test 1 group received EP 30 min, 12, 24, 36, 48, 60 and 72 h post-injury, and the Test 2 group was treated with a single dose of EP 30 min post-thermal injury. The control group received a single intraperitoneal (IP) injection of an equivalent volume of normal saline solution (0.9%). On the 3rd and 21st days after the injury, five rats from each group were euthanized. Macroscopic, histopathological and immunohistochemical assessments were performed along with biochemical evaluations that included malondialdehyde (MDA), total antioxidant capacity (TAC) and HP measurements.
The positive effects of EP on reducing and controlling oxidative stress, inflammation and death were achieved. Generally, inflammation control further preservation of the stasis zone and accelerated healing in the coagulation area were also proven.
This study revealed that administering EP through injection can effectively halt the ongoing tissue necrosis in the stasis zone and significantly promote wound healing, primarily due to its anti-inflammatory and antioxidant properties.
由于过度的氧化应激、炎症和进行性组织坏死，烧伤是一项重大的临床挑战，会导致愈合延迟和功能障碍。丙酮酸乙酯（EP）已被证明具有强大的抗炎、抗氧化、抗凋亡和抗纤维化特性，使其成为治疗烧伤创面的一种有前景的治疗药物。本研究旨在评估EP促进烧伤创面愈合和预防组织坏死的疗效。
在诱导热损伤后，将30只雄性Wistar大鼠随机分为三个不同的组。试验1组在伤后30分钟、12、24、36、48、60和72小时接受EP治疗，试验2组在热损伤后30分钟接受单剂量EP治疗。对照组腹腔内注射等量的生理盐水溶液（0.9%）。在伤后第3天和第21天，每组处死5只大鼠。进行宏观、组织病理学和免疫组织化学评估以及包括丙二醛（MDA）、总抗氧化能力（TAC）和HP测量在内的生化评估。
EP在减少和控制氧化应激、炎症和死亡方面取得了积极效果。一般来说，炎症控制、进一步保留淤血区以及加速凝血区的愈合也得到了证实。
本研究表明，通过注射给予EP可以有效阻止淤血区正在进行的组织坏死，并显著促进伤口愈合，这主要归功于其抗炎和抗氧化特性。
