França Renata Cristina Sobreira, Dias Rebeca Tibau Aguiar, Reis Ranam Moreira, Sousa Frederico Barbosa de, Carlo Hugo Lemes, Santos Rogerio Lacerda Dos, Carvalho Fabíola Galbiatti de
Universidade Federal da Paraíba (UFPB), Programa de Pós-Graduação em Odontologia, João Pessoa, Paraíba, Brasil.
Universidade Estadual de Campinas (UNICAMP), Programa de Pós-Graduação em Odontologia, Piracicaba, São Paulo, Brasil.
J Appl Oral Sci. 2025 Apr 18;33:e20240445. doi: 10.1590/1678-7757-2024-0445. eCollection 2025.
Chitosan nanoparticles suspension (ChNPs) showed antimicrobial effects in the oral cavity, but its effects on enamel erosion prevention remain little explored.
This study synthesized ChNPs and evaluated their effect on enamel after erosive challenge.
ChNPs were synthesized by ionic gelation and characterized using scanning electron microscopy (SEM), infrared spectrophotometry (FTIR), dynamic light scattering methods (DLS) and zeta potential (ZP). In total, 56 human enamel blocks were divided into four groups (n=14/group): (i) ChNPs suspension (4.4mg/mL); (ii) 0.05% sodium fluoride solution (NaF); (iii) chitosan solution (5.0 mg/mL); and (iv) distilled water. After incubation in freshly collected human saliva (3h), the samples were exposed to erosive challenge in 1% citric acid (90s) and remineralizing solution (2h) performed four times a day. After the 1st and 4th acid exposures, solutions were applied for 2 min. After 7 days, % Vickers surface hardness change (% SMH) was obtained using 300 g load applied for 15s. Enamel surface loss was evaluated using optical profilometer by subtracting the final profile values from baseline ones. Data were analyzed by ANOVA and post-hoc T tests (α=0.05). Surface topography was obtained by optical profilometer.
SEM revealed the formation of spherical nanoparticles. DLS showed nanoparticles with 85.7±10.5 nm diameter and ZP value of +45.5±5.4mV. Enamel surface loss was significantly lower in ChNPs and NaF groups, exhibiting a less rough surface in the treated areas. ChNPs, NaF and chitosan groups showed lower % SMH values.
ChNPs suspension minimized enamel loss after in vitro erosive challenge and appears to be a promising material for enamel erosion prevention.
壳聚糖纳米颗粒混悬液（ChNPs）在口腔中显示出抗菌作用，但其对预防牙釉质侵蚀的作用仍鲜有人研究。
本研究合成ChNPs并评估其在侵蚀性刺激后对牙釉质的影响。
通过离子凝胶法合成ChNPs，并使用扫描电子显微镜（SEM）、红外分光光度法（FTIR）、动态光散射法（DLS）和zeta电位（ZP）对其进行表征。总共56个人类牙釉质块被分为四组（每组n = 14）：（i）ChNPs混悬液（4.4mg/mL）；（ii）0.05%氟化钠溶液（NaF）；（iii）壳聚糖溶液（5.0mg/mL）；（iv）蒸馏水。在新鲜采集的人类唾液中孵育3小时后，将样本暴露于1%柠檬酸中进行侵蚀性刺激（90秒），并每天进行4次再矿化溶液处理（2小时）。在第1次和第4次酸暴露后，每种溶液处理2分钟。7天后，使用300g载荷施加15秒来获得维氏表面硬度变化百分比（%SMH）。通过光学轮廓仪减去最终轮廓值与基线值来评估牙釉质表面损失。数据通过方差分析和事后T检验进行分析（α = 0.05）。通过光学轮廓仪获得表面形貌。
SEM显示形成了球形纳米颗粒。DLS显示纳米颗粒直径为85.7±10.5nm，ZP值为+45.5±5.4mV。ChNPs组和NaF组的牙釉质表面损失显著更低，处理区域的表面粗糙度更小。ChNPs组、NaF组和壳聚糖组的%SMH值更低。
ChNPs混悬液在体外侵蚀性刺激后使牙釉质损失最小化，似乎是一种有前景的预防牙釉质侵蚀的材料。
