Luo Lanqing, Kuai Rui
School of Pharmaceutical Sciences, Tsinghua University, Beijing, China.
Tsinghua-Peking Center for Life Sciences, Beijing, China.
Methods Mol Biol. 2025;2926:129-141. doi: 10.1007/978-1-0716-4542-0_10.
Efficient delivery of tumor antigen peptides and adjuvants to dendritic cells in lymphoid organs is critical for eliciting potent tumor antigen-specific CD8+ T cell responses. However, soluble antigens and adjuvants often suffer from rapid clearance and fail to induce strong T-cell responses. Synthetic high-density lipoproteins (sHDL) nanodiscs, which are 10-nm discoidal nanoparticles, have been shown to facilitate the co-delivery of peptide antigens and adjuvants to lymph nodes and induce strong and durable antigen presentation by dendritic cells, which markedly improve the overall magnitude of antigen-specific T cell responses. Here we summarize the methods for preparing sHDL-based peptide cancer vaccines and protocols for analyzing the T cell responses against different tumor antigens. We envision that these methods will be helpful in developing and assessing other nanoparticle-based peptide cancer vaccines in the future.
将肿瘤抗原肽和佐剂有效递送至淋巴器官中的树突状细胞，对于引发强大的肿瘤抗原特异性CD8+ T细胞反应至关重要。然而，可溶性抗原和佐剂常常会迅速清除，无法诱导强烈的T细胞反应。合成高密度脂蛋白（sHDL）纳米盘是直径为10纳米的盘状纳米颗粒，已被证明可促进肽抗原和佐剂共同递送至淋巴结，并诱导树突状细胞进行强大且持久的抗原呈递，这显著提高了抗原特异性T细胞反应的整体强度。在此，我们总结了基于sHDL的肽癌症疫苗的制备方法以及分析针对不同肿瘤抗原的T细胞反应的方案。我们设想，这些方法将有助于未来开发和评估其他基于纳米颗粒的肽癌症疫苗。
