用于定量实时PCR的参考基因的选择

Selection of Reference Genes for Quantitative Real-Time PCR.

作者信息

Zhou Ting, Feng Huahua, Zhang Jie, Tang Yanlong, Dong Xiaoling, Kang Kui

机构信息

MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-Construction by Ministry and Province), College of Agriculture, Yangtze University, Jingzhou 434025, China.

College of Biology and Agriculture, Zunyi Normal University, Zunyi 563006, China.

出版信息

Insects. 2025 Mar 4;16(3):268. doi: 10.3390/insects16030268.

DOI:10.3390/insects16030268

PMID:40266757

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11943240/

Abstract

is a newly discovered species of parasitoid wasps. Research into its development, behavioral genetics, and molecular mechanisms provides valuable insights for improving integrated pest management strategies. Quantitative real-time PCR (qRT-PCR) is the most commonly used method for analyzing gene expression. This method requires the identification of stable reference genes to accurately evaluate transcriptional level variations. In this study, eight candidate reference genes (, , , , , , , and ) were identified and evaluated for their suitability as reference genes. Gene expression levels across different developmental stages were analyzed using three software tools, GeNorm, NormFinder, and BestKeeper, and the online tool RefFinder. The overall ranking of reference gene stability was as follows: > > > > > > > . Ultimately, was determined to be the most stable reference gene.

摘要

是一种新发现的寄生蜂物种。对其发育、行为遗传学和分子机制的研究为改进害虫综合治理策略提供了有价值的见解。定量实时PCR（qRT-PCR）是分析基因表达最常用的方法。该方法需要鉴定稳定的内参基因，以准确评估转录水平的变化。在本研究中，鉴定并评估了八个候选内参基因（、、、、、、和）作为内参基因的适用性。使用三种软件工具GeNorm、NormFinder和BestKeeper以及在线工具RefFinder分析了不同发育阶段的基因表达水平。内参基因稳定性的总体排名如下：>>> >>> >>> >>> >>> >>> 。最终，被确定为最稳定的内参基因。