State Key Laboratory of Rice Biology and Breeding, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310058, China.
Zhejiang University Zhongyuan Institute, Zhengzhou, 450000, China.
Sci Data. 2024 May 2;11(1):438. doi: 10.1038/s41597-024-03278-0.
The Bethylidae are the most diverse of Hymenoptera chrysidoid families. As external parasitoids, the bethylids have been widely adopted as biocontrol agents to control insect pests worldwide. Thus far, the genomic information of the family Bethylidae has not been reported yet. In this study, we crystallized into a high-quality chromosome-level genome of ant-like bethylid wasps Sclerodermus sp. 'alternatusi' (Hymenoptera: Bethylidae) using PacBio sequencing as well as Hi-C technology. The assembled S. alternatusi genome was 162.30 Mb in size with a contig N50 size of 3.83 Mb and scaffold N50 size of 11.10 Mb. Totally, 92.85% assembled sequences anchored to 15 pseudo-chromosomes. A total of 10,204 protein-coding genes were annotated, and 23.01 Mb repetitive sequences occupying 14.17% of genome were pinpointed. The BUSCO results showed that 97.9% of the complete core Insecta genes were identified in the genome, while 97.1% in the gene sets. The high-quality genome of S. alternatusi will not only provide valuable genomic information, but also show insights into parasitoid wasp evolution and bio-control application in future studies.
长尾蜾蠃科是膜翅目金小蜂科中最多样化的科。长尾蜾蠃作为外寄生蜂，已被广泛用作生物防治剂来控制世界各地的害虫。迄今为止，还没有报道过长尾蜾蠃科的基因组信息。在这项研究中，我们使用 PacBio 测序和 Hi-C 技术对蚁形长尾蜾蠃 Sclerodermus sp. 'alternatusi'（膜翅目：长尾蜾蠃科）进行了高质量染色体水平的基因组结晶。组装的 S. alternatusi 基因组大小为 162.30 Mb，contig N50 大小为 3.83 Mb，scaffold N50 大小为 11.10 Mb。总共，92.85%的组装序列锚定在 15 个假染色体上。总共注释了 10,204 个蛋白质编码基因，并确定了 23.01 Mb 重复序列，占基因组的 14.17%。BUSCO 结果表明，在基因组中鉴定出了完整核心昆虫基因的 97.9%，在基因集中鉴定出了 97.1%。高质量的 S. alternatusi 基因组不仅将提供有价值的基因组信息，而且还将为寄生蜂的进化和未来生物防治应用提供深入了解。
