Singh Yazavinder, Zotte Antonella Dalle, Cullere Marco, Chundang Pipatpong, Kongsup Penpicha, Kovitvadhi Attawit
Department of Animal Medicine, Production and Health, University of Padova, Agripolis, Viale dell'Università 16, Legnaro, 35020 Padova, Italy.
Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
Vet Sci. 2025 Mar 15;12(3):277. doi: 10.3390/vetsci12030277.
The present study investigated the in vitro digestibility of diets for broiler chickens (Ross308) and broiler and laying Japanese quails (). The diets contained unconventional feedstuffs such as silkworm () meal (SWM) and meals obtained from different lines (Pearl and Alan, characterized by reduced linoleic acid and glucosinolates content, respectively). An in vitro technique was tested in order to assess its potential for replacing in vivo studies. To test this, the digestive tracts of fifty broiler chickens and four hundred Japanese quails were sampled to extract digestive enzymes to be used for in vitro digestibility assessments, including dry matter digestibility (DMd), organic matter digestibility (OMd), and crude protein digestibility (CPd). Diets including SWM exhibited comparable digestibility values to those of Control diet for broiler chickens, highlighting its potential as a valuable protein source in poultry nutrition. The study also found strong correlations between DMd and OMd ( < 0.01), indicating a logical relationship in nutrient breakdown. A 5% or 10% inclusion of the two camelina lines in the diet for broiler quails did not significantly alter the digestibility parameters ( ≥ 0.05), whereas in laying quails, diets with a 15% inclusion level of camelina resulted in a significant difference in digestibility ( < 0.05). Specifically, oil diets provided the best outcomes, while the diet Pearl Spring 15 showed the lowest DMd, CPd, and OMd ( < 0.05). Overall, results from the present study indicate that the tested alternative feedstuffs (SWM and camelina) have a good potential for poultry feed formulations. In addition, the tested in vitro technique was shown to be more suitable to predict the digestibility of single feedstuffs (i.e., SWM) rather than complete diets, which is consistent with the existing literature. For this reason, this in vitro technique is not adequate to replace in vivo digestibility experiments.
本研究调查了肉鸡（罗斯308）以及肉用和蛋用日本鹌鹑日粮的体外消化率。日粮中含有非常规饲料原料，如蚕蛹粉（SWM）以及来自不同亚麻荠品系（珍珠和艾伦，分别具有较低的亚油酸和硫代葡萄糖苷含量）的粕类。测试了一种体外技术，以评估其替代体内研究的潜力。为此，采集了50只肉鸡和400只日本鹌鹑的消化道，提取消化酶用于体外消化率评估，包括干物质消化率（DMd）、有机物消化率（OMd）和粗蛋白消化率（CPd）。含SWM的日粮对肉鸡而言，其消化率值与对照日粮相当，突出了其在家禽营养中作为优质蛋白质来源的潜力。该研究还发现DMd和OMd之间存在强相关性（<0.01），表明在养分分解方面存在逻辑关系。在肉用鹌鹑日粮中添加5%或10%的这两个亚麻荠品系，并未显著改变消化率参数（≥0.05），而在蛋用鹌鹑中，亚麻荠添加水平为'15%的日粮在消化率方面产生了显著差异（<0.05）。具体而言，油粕日粮效果最佳，而珍珠泉15日粮的DMd、CPd和OMd最低（<0.05）。总体而言，本研究结果表明，所测试的替代饲料原料（SWM和亚麻荠）在禽类饲料配方中具有良好潜力。此外，所测试的体外技术更适合预测单一饲料原料（即SWM）的消化率，而非完整日粮的消化率，这与现有文献一致。因此，这种体外技术不足以替代体内消化率实验。
