Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.
Department of Applied Science, Faculty of Science, Prince of Songkla University, Songkhla, Thailand.
J Anim Physiol Anim Nutr (Berl). 2021 Mar;105(2):305-315. doi: 10.1111/jpn.13451. Epub 2020 Sep 15.
The objective of this study is to identify potential insect species comparing with commonly used protein sources based on efficiency of the in vitro digestibility on dry matter (DMd), organic matter (OMd) and crude protein (CPd) in broiler chickens, black-meat chickens (Native breed) and quails. Each of gastric mucosa, pancreas and duodenal mucosa were obtained from proventriculus, pancreas and duodenum, respectively. Crude digestive enzyme extracts (CTE) were extracted from these organs to perform in vitro digestibility. Eighteen insect samples and six commonly used protein sources were served as substrates which were evaluated on DMd, OMd and CPd in triplicate for each substrate. The CTE from gastric mucosa was used to simulate proventriculus, whereas small intestine was simulation by adding the CTE from pancreas and duodenum. The large variation of chemical composition between insect meals was presented. For commonly used protein sources, animal proteins were higher on digestibility than plant proteins (p < .001). Quails represented a great potential to digest insect meals comparing other animals. Based on CPd results, there were potential insect species for broiler chickens (Achroia grisella: AG, Tenebrio molitor: TM and Musca domestica), black-meat chickens (Patanga succincta, TM and AG) and quails (Hermetia illucens, Acheta domesticus and Locusta migratoria; p < .001). The evidences from this study suggest that these insect species contain a great potential to use as alternative protein sources promoting an animal production with sustainability. However, the in vivo experimentation must be performed to confirm in further study.
本研究旨在基于体外消化率比较常用蛋白质源的潜在昆虫种类，体外消化率在肉鸡、黑肉鸡（本地品种）和鹌鹑中对干物质（DMd）、有机物（OMd）和粗蛋白（CPd）的影响。分别从前胃、胰腺和十二指肠中获得胃黏膜、胰腺和十二指肠黏膜。从这些器官中提取粗消化酶提取物（CTE）以进行体外消化率测试。18 种昆虫样本和 6 种常用蛋白质源作为底物，每种底物重复 3 次评估 DMd、OMd 和 CPd。胃黏膜的 CTE 用于模拟前胃，而胰腺和十二指肠的 CTE 用于模拟小肠。昆虫餐的化学成分变化很大。对于常用的蛋白质源，动物蛋白的消化率高于植物蛋白（p<0.001）。与其他动物相比，鹌鹑对昆虫餐有很大的消化潜力。根据 CPd 结果，肉鸡（Achroia grisella：AG、Tenebrio molitor：TM 和 Musca domestica）、黑肉鸡（Patanga succincta、TM 和 AG）和鹌鹑（Hermetia illucens、Acheta domesticus 和 Locusta migratoria 都有潜在的昆虫种类（p<0.001）。本研究的证据表明，这些昆虫种类具有很大的潜力可作为替代蛋白质源，促进可持续的动物生产。然而，必须进行体内实验以在进一步研究中确认。
