Löw P, Peterson E, Mizuno M, Takigawa T, Chojnacki T, Dallner G
Biochem Biophys Res Commun. 1985 Jul 16;130(1):460-6. doi: 10.1016/0006-291x(85)90439-5.
Chemical synthesis was used to produce optically active isomers of dolichol (S- and R-forms) with 18 and 19 isoprene residues. The phosphorylated polyprene was studied in rat liver microsomal GDP-mannosyl and UDP-N-acetylglucosaminyl transferase systems. The two dolichol-P forms in both transferase systems gave Vmax values which for the S-form exceeded 4-6 times what was obtained with the R-form. The Km values were also higher for the S-form. The hepatocyte appears to contain a large excess of dolichyl-P, by 100 times exceeding that of the Km values. For this reason the S-form of dolichyl-P seems to be one of the requirements for the normal establishment of the N-glycosidically linked oligosaccharide chain.
采用化学合成法制备了含有18个和19个异戊二烯残基的多萜醇光学活性异构体（S型和R型）。在大鼠肝微粒体GDP-甘露糖基转移酶和UDP-N-乙酰葡糖胺基转移酶系统中对磷酸化多萜烯进行了研究。在这两种转移酶系统中，两种多萜醇磷酸酯形式的Vmax值表明，S型比R型高出4至6倍。S型的Km值也更高。肝细胞中似乎含有大量过量的多萜醇磷酸酯，比Km值高出100倍。因此，多萜醇磷酸酯的S型似乎是正常形成N-糖苷连接寡糖链的必要条件之一。
