Su Yun, He Wanzhuo, Zheng Lei, Fan Xianqun, Hu Tony Y
Department of Ophthalmology, Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P. R. China.
Department of Laboratory Medicine, Guangdong Provincial Key Laboratory of Precision Medical Diagnostics, Guangdong Engineering and Technology Research Center for Rapid Diagnostic Biosensors, Guangdong Provincial Key Laboratory of Single Cell Technology and Application, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P. R. China.
ACS Nano. 2025 May 6;19(17):16193-16203. doi: 10.1021/acsnano.5c00705. Epub 2025 Apr 24.
Single extracellular vesicle (EV) research holds the potential to revolutionize our understanding of cellular communication and enable breakthroughs in diagnostics and therapeutics. However, the lack of a clear, consensus-driven definition of single EV research has led to methodological inconsistencies, overgeneralized interpretations, and, in some cases, misleading claims. In this perspective, we propose a framework for defining single EV research, critique current challenges and misconceptions in this field, and discuss its implications for biomedical applications. We argue that precise experimental design, rigorous validation, and interdisciplinary collaboration approaches are needed to establish single EV research as a cornerstone of precision medicine.
单细胞外囊泡（EV）研究有潜力彻底改变我们对细胞通讯的理解，并在诊断和治疗方面取得突破。然而，缺乏一个明确的、由共识驱动的单细胞外囊泡研究定义，导致了方法上的不一致、过度概括的解释，在某些情况下还出现了误导性的说法。从这个角度来看，我们提出了一个定义单细胞外囊泡研究的框架，批评了该领域当前的挑战和误解，并讨论了其对生物医学应用的影响。我们认为，需要精确的实验设计、严格的验证和跨学科合作方法，才能将单细胞外囊泡研究确立为精准医学的基石。
