Netesova M A, Shornikova M V, Chentsev Iu S
Nauchnye Doki Vyss Shkoly Biol Nauki. 1985(5):55-61.
It has been shown that the morphofunctional alterations of nucleoli in KEPV cells induced by actinomycin D at 0,05 microgram/ml proceed in two steps. During the first 3 h of actinomycin D action a level of the RNA synthesis in nucleoli falls to 25% from the initial one. Simultaneously the following morphological alterations take place: condensation of the chromatin, infiltration of nucleoli, displacement to outlining regions, increase of the dimensions and reduction of number of fibrillar centres, gradual disappearance of compact fibrillar zone and appearance looplike granular structures. During the following 3-6 h of antibiotic action the RNA synthesis falls slightly, nucleoli dimensions decrease, fibrillar micronucleoli appear.
已表明，在0.05微克/毫升放线菌素D诱导下，KEPV细胞中核仁的形态功能改变分两个阶段进行。在放线菌素D作用的最初3小时内，核仁中的RNA合成水平从初始水平降至25%。同时发生以下形态学改变：染色质凝聚、核仁浸润、向周边区域移位、纤维中心尺寸增加和数量减少、致密纤维区逐渐消失以及出现环状颗粒结构。在抗生素作用的接下来3 - 6小时内，RNA合成略有下降，核仁尺寸减小，出现纤维状微核仁。
