Dudnik O A, Zatsepina O V, Chentsov Iu S
Tsitologiia. 1993;35(5):10-6.
The effect of a 20% Hanks [correction of Hanx] solution on living cells of pig embryo kidney culture induces sharp changes in the structural and functional organization of nucleoli. 10-15 min after the beginning of the effect, no nucleoli are observed under the light microscope. The ultrathin sections reveal only fibrillar centers (FC) and a dense fibrillar component. Under these conditions nucleoli do not incorporate 3H-uridine. However, 30-120 min after the cells are transferred from the hypotonic to the normal isotonic medium, the nucleoli cannot be distinguished from initial ones by their form, size, location in the nucleus, ultrastructure and the 3H-uridine labelling pattern. This indicates that the effect of a 20% Hanks [correction of Hanx] solution on nucleoli of living PE cells is reversible. It has been suggested that reconstruction of nucleoli in the full culture medium may proceed at the expense of both synthesis de novo and the material of initial nucleoli.
20% 汉克斯溶液对猪胚胎肾培养的活细胞的作用会引起核仁结构和功能组织的急剧变化。作用开始后10 - 15分钟，在光学显微镜下观察不到核仁。超薄切片仅显示纤维中心（FC）和致密纤维成分。在这些条件下，核仁不掺入3H - 尿苷。然而，在细胞从低渗培养基转移到正常等渗培养基后30 - 120分钟，核仁在形态、大小、在细胞核中的位置、超微结构和3H - 尿苷标记模式方面与初始核仁无法区分。这表明20% 汉克斯溶液对活的猪胚胎肾细胞的核仁的作用是可逆的。有人提出，在完全培养基中核仁的重建可能以从头合成和初始核仁的物质为代价进行。
