Wei Chunyan, Yang Huizhen, Ye Bingbing, Wei Wei, Shan Wei, Chen Jianye, Chen Kunsong, Li Xian, Deng Zhiping, Zhang Bo
Zhejiang Key Laboratory of Horticultural Crop Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Desheng Middle Road No. 298, Hangzhou, Zhejiang Province 310021, China.
Plant Physiol. 2025 Apr 30;198(1). doi: 10.1093/plphys/kiaf159.
Plant secondary metabolites undergo changes in response to UV-B irradiation. Although UV-B irradiation reduces flavor-associated volatile compounds in detached peach (Prunus persica L. Batsch) fruit, the underlying regulatory mechanisms remain unclear. By integrating proteomic, transcriptomic, and metabolomic data from peach fruit following UV-B irradiation, we discovered that the detached fruit responds to UV-B by suppressing the biosynthesis of the flavor-related monoterpene linalool. We identified PpMADS2, a transcription factor that regulates linalool biosynthesis by activating terpene synthase 1 (PpTPS1) expression. PpMADS2 overexpression in peach and tomato fruits significantly increased linalool levels compared with the controls. Proteomic data and immunoblots revealed a decrease in PpMADS2 abundance following exposure to UV-B. Moreover, our results demonstrated that PpMADS2 interacts with the E3 ubiquitin ligase PpCOP1 both in vitro and in vivo. The UV-B-induced 26S-proteasome-mediated degradation of PpMADS2 is largely PpCOP1-dependent. Taken together, our findings demonstrate that linalool biosynthesis in detached peach fruit exposed to UV-B radiation is governed by the PpCOP1-PpMADS2-PpTPS1 module. This study enhances our understanding of the interplay between light signaling and fruit flavor quality. Multiomics approaches offer valuable resources for investigating the mechanisms underlying how light influences metabolism in fruit crops.
植物次生代谢产物会因紫外线B（UV-B）辐射而发生变化。尽管UV-B辐射会降低离体桃（Prunus persica L. Batsch）果实中与风味相关的挥发性化合物含量，但其潜在调控机制仍不清楚。通过整合UV-B辐射后桃果实的蛋白质组学、转录组学和代谢组学数据，我们发现离体果实通过抑制风味相关单萜芳樟醇的生物合成来响应UV-B。我们鉴定出PpMADS2，一种通过激活萜烯合酶1（PpTPS1）表达来调控芳樟醇生物合成的转录因子。与对照相比，桃和番茄果实中PpMADS2的过表达显著提高了芳樟醇水平。蛋白质组学数据和免疫印迹显示，暴露于UV-B后PpMADS2丰度降低。此外，我们的结果表明，PpMADS2在体外和体内均与E3泛素连接酶PpCOP1相互作用。UV-B诱导的26S蛋白酶体介导的PpMADS2降解在很大程度上依赖于PpCOP1。综上所述，我们的研究结果表明，暴露于UV-B辐射下的离体桃果实中芳樟醇的生物合成受PpCOP1-PpMADS2-PpTPS1模块调控。本研究增进了我们对光信号与果实风味品质之间相互作用的理解。多组学方法为研究光影响果树作物代谢的潜在机制提供了有价值的资源。
