Department of Medical Biotechnology, Yeungnam University, Gyeongsan 38541, Korea.
Research Institute of Cell Culture, Yeungnam University, Gyeongsan 38541, Korea.
Int J Mol Sci. 2021 Jan 16;22(2):876. doi: 10.3390/ijms22020876.
Skeletal muscle is the most abundant tissue and constitutes about 40% of total body mass. Herein, we report that crude water extract (CWE) of enhanced myoblast proliferation and differentiation. Pretreatment of mice with the CWE of prior to cardiotoxin-induced muscle injury was found to enhance muscle regeneration by inducing myogenic gene expression and downregulating myostatin expression. Furthermore, this extract reduced nitrotyrosine protein levels and atrophy-related gene expression. Of the five different fractions of the CWE of obtained, the ethyl acetate (EtOAc) fraction more significantly enhanced myoblast proliferation and differentiation than the other fractions. Ten bioactive compounds were isolated from the EtOAc fraction and characterized by GC-MS and NMR. Of these compounds (4-hydroxybenzoic acid, liquiritigenin, ()-(-)-vestitol, isoliquiritigenin, medicarpin, tetrahydroxymethoxychalcone, licochalcone B, liquiritin, liquiritinapioside, and ononin), liquiritigenin, tetrahydroxymethoxychalcone, and licochalcone B were found to enhance myoblast proliferation and differentiation, and myofiber diameters in injured muscles were wider with the liquiritigenin than the non-treated one. Computational analysis showed these compounds are non-toxic and possess good drug-likeness properties. These findings suggest that G. uralensis-extracted components might be useful therapeutic agents for the management of muscle-associated diseases.
骨骼肌是最丰富的组织，占人体总质量的 40%左右。在此，我们报告称， 的粗水提取物（CWE）可增强成肌细胞的增殖和分化。在心肌毒素诱导的肌肉损伤前用 CWE 预处理小鼠，发现可通过诱导肌生成基因表达和下调肌肉生长抑制素表达来增强肌肉再生。此外，这种提取物还降低了硝基酪氨酸蛋白水平和与萎缩相关的基因表达。在 的五种不同 CWE 馏分中，乙酸乙酯（EtOAc）馏分比其他馏分更显著地增强了成肌细胞的增殖和分化。从 EtOAc 馏分中分离出 10 种生物活性化合物，并通过 GC-MS 和 NMR 进行了表征。在这些化合物（对羟基苯甲酸、甘草素、()-(-)-vestitol、异甘草素、甘草查尔酮 A、甘草次酸、四羟甲氧基查尔酮、甘草酮 B、甘草素、甘草素-7-O-β-D-吡喃葡萄糖苷和大豆苷元）中，甘草素、四羟甲氧基查尔酮和甘草酮 B 被发现可增强成肌细胞的增殖和分化，并且用甘草素处理后的受损肌肉中的肌纤维直径比未处理的更宽。计算分析表明这些化合物无毒且具有良好的药物样特性。这些发现表明，甘草提取物中的成分可能是治疗与肌肉相关疾病的有用治疗剂。
