Etzold Christian, Lyros Orestis, Mehdorn Matthias, Nowotny Robert, Niebisch Stefan, Jansen-Winkeln Boris, Schierle Katrin, Gockel Ines, Thieme René
Department of Visceral, Transplant, Thoracic and Vascular Surgery, University Hospital of Leipzig, Leipzig, Germany.
Department of General, Visceral and Oncological Surgery, St. Georg Hospital Leipzig, Leipzig, Germany.
Pleura Peritoneum. 2025 Feb 26;10(1):1-9. doi: 10.1515/pp-2024-0012. eCollection 2025 Mar.
The prognosis of patients with peritoneal metastases (PM) is poor, and these patients have a brief overall survival. Most patients with advanced PM receive palliative therapy to maintain their quality of life. In our current study, we investigated whether patient-specific 3D-tissue slices from patients with PM subjected to pressurized intraperitoneal aerosol chemotherapy could be cultured .
Biopsies from gastric cancer patients with PM were characterized for cytokeratin-positive tumor cells and the proliferation marker Ki-67. Biopsies from seven patients were cut to 350 µM thick slices in a standardized manner, cultured with 10 µM 5-fluorouracil, doxorubicin, cisplatin, oxaliplatin, or irinotecan for 96 h, and then examined histopathologically and via immunohistochemistry for persistent cytokeratin and Ki-67 expression.
cultured slices revealed a similar morphology to un-cultured specimens, and Ki-67-positive tumor cell areas were present after 96 h. The total amount of tumor cells per slice was determined by pan-cytokeratin staining. In the doxorubicin-treated slices, the cytokeratin-positive tumor cell fraction and proliferative (Ki-67pos) cells were decreased. Patient-specific 3D-tissue-slice cultures from peritoneal biopsies were cultured for up to 4 days.
Potentially, these cultures are a reliable model to evaluate the chemosensitivity of patients with PM. Further investigation is needed to match the chemosensitivity with the clinical course of these patients.
腹膜转移（PM）患者预后较差，总生存期较短。大多数晚期PM患者接受姑息治疗以维持生活质量。在我们当前的研究中，我们调查了接受腹腔加压气雾化疗的PM患者的个体化三维组织切片是否能够进行培养。
对患有PM的胃癌患者的活检组织进行细胞角蛋白阳性肿瘤细胞和增殖标志物Ki-67的鉴定。将7例患者的活检组织以标准化方式切成350µM厚的切片，用10µM 5-氟尿嘧啶、阿霉素、顺铂、奥沙利铂或伊立替康培养96小时，然后通过组织病理学和免疫组织化学检查持续的细胞角蛋白和Ki-67表达。
培养的切片显示出与未培养标本相似的形态，96小时后存在Ki-67阳性肿瘤细胞区域。通过全细胞角蛋白染色确定每片肿瘤细胞的总量。在阿霉素处理的切片中，细胞角蛋白阳性肿瘤细胞分数和增殖性（Ki-67阳性）细胞减少。来自腹膜活检的患者个体化三维组织切片培养长达4天。
这些培养物有可能是评估PM患者化疗敏感性的可靠模型。需要进一步研究以将化疗敏感性与这些患者的临床病程相匹配。
