Yang Hao, Hua Yuqi, Liu Tao, Fang Siming, Gu Wei, Shi Juan
Beijing Key Lab Forest Pest Control, Beijing Forestry University, Beijing, China.
Coll Forestry, Sino French Joint Lab Invasive Forest Pests Eurasia, Beijing Forestry University, Beijing, China.
Pest Manag Sci. 2025 Aug;81(8):4611-4620. doi: 10.1002/ps.8821. Epub 2025 Apr 25.
Pine wilt disease (PWD), caused by the pine wood nematode (PWN) Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle, poses a significant global threat to pine forests. This study investigated the nematicidal efficacy of four fumigants-methyl bromide (MB), phosphine (PH), sulfuryl fluoride (SF), and methyl isothiocyanate (MITC)-applied both individually and in combination (PH+MB, SF+MB, MITC+MB, PH+SF, PH+MITC, and MITC+SF) against PWN.
Under uniform fumigation conditions, PH, SF, and MITC demonstrated nematicidal effects comparable with MB. Furthermore, the combination of these fumigants with MB exhibited synergistic effects in eradicating PWN. Compared with treatments with individual fumigants, incorporating small amounts of PH, SF, or MITC reduced the required MB dosage by at least 60% to achieve the same mortality rate. Optimal efficiency was observed with ratios of 1:9 for PH:MB, 1:9 for SF:MB, and 3:7 for MITC:MB. Although the combination of PH with SF showed an insignificant synergistic effect, the combination of MITC with either PH or SF exhibited pronounced synergy, with the optimal synergistic ratios being 1:9 for PH:MITC and 5:5 for SF:MITC.
This study highlights the efficacy of PH, SF, and MITC as potential alternatives to MB for controlling PWN. Moreover, the strong synergistic interactions observed when combining PH, SF, or MITC with MB offer a promising strategy to reduce MB dependency. Furthermore, the notable synergy between MITC and PH or SF further supports their utility in integrated fumigation approaches for PWN management. © 2025 Society of Chemical Industry.
松材线虫（PWN）松材线虫（Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle）引起的松树萎蔫病对全球松林构成重大威胁。本研究调查了四种熏蒸剂——甲基溴（MB）、磷化氢（PH）、硫酰氟（SF）和甲基异硫氰酸酯（MITC）——单独使用以及组合使用（PH+MB、SF+MB、MITC+MB、PH+SF、PH+MITC和MITC+SF）对松材线虫的杀线虫效果。
在均匀熏蒸条件下，PH、SF和MITC表现出与MB相当的杀线虫效果。此外，这些熏蒸剂与MB组合在根除松材线虫方面表现出协同作用。与单独使用熏蒸剂的处理相比，加入少量的PH、SF或MITC可将达到相同死亡率所需的MB剂量至少降低60%。当PH:MB比例为1:9、SF:MB比例为1:9、MITC:MB比例为3:7时观察到最佳效果。虽然PH与SF的组合显示出不显著的协同作用，但MITC与PH或SF的组合表现出明显的协同作用，最佳协同比例为PH:MITC为1:9，SF:MITC为5:5。
本研究强调了PH、SF和MITC作为控制松材线虫的MB潜在替代品的有效性。此外，PH、SF或MITC与MB组合时观察到的强协同相互作用提供了一种有前景的策略来减少对MB的依赖。此外，MITC与PH或SF之间显著的协同作用进一步支持了它们在松材线虫综合熏蒸管理方法中的实用性。© 2025化学工业协会。
