Wishart G J
Br Poult Sci. 1985 Jul;26(3):375-80. doi: 10.1080/00071668508416825.
The fertilising abilities of freshly-diluted and frozen and thawed fowl semen were compared by inseminating a wide range (0.2 to 1600 X 10(6] of doses of spermatozoa. These data demonstrated that there was a non-linear relationship between numbers of spermatozoa and the probability of fertilisation. It was concluded that a typical fertility trial, which assesses the percentage of fertile eggs laid by groups of hens inseminated with a fixed dose of semen, was inadequate for comparing the quality of semen samples. An alternative fertility trial is described, which involves assessing the numbers of spermatozoa required to fertilize a particular percentage of eggs laid. This modified method showed that the quality of frozen and thawed semen, in terms of its fertilising ability, was reduced to 1.6% of that of fresh semen.
通过授精不同剂量范围（0.2至1600×10⁶）的精子，比较了新鲜稀释以及冷冻解冻后的家禽精液的受精能力。这些数据表明，精子数量与受精概率之间存在非线性关系。得出的结论是，典型的受精试验（评估用固定剂量精液授精的母鸡群体所产受精卵的百分比）不足以比较精液样本的质量。本文描述了一种替代的受精试验，该试验涉及评估使特定百分比的所产蛋受精所需的精子数量。这种改进方法表明，冷冻解冻后精液的受精能力质量降至新鲜精液的1.6%。
