Wu Lichun, Zhou Yu, Zhang Yi, Hu Jia, Ikegami Yasuhiro, Aishima Shinichi, Ijima Hiroyuki
Department of Chemical Engineering, Faculty of Engineering, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Institute for Materials Chemistry and Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Gels. 2025 Apr 3;11(4):266. doi: 10.3390/gels11040266.
As dressings for moist wound healing, hyaluronic acid hydrogels play a significant role in maintaining moisture and promoting wound healing. However, existing hydrogel dressings are inadequate in terms of slow gelation time, weak mechanical performance, and fast degradation, which increases the risk of secondary infections during treatment. Therefore, we developed a hyaluronic acid double network hydrogel (DNH). Compared to single-network hydrogels (hydrazone and Diels-Alder), DNH shows a short gelation time (25 s) and strong mechanical properties (Young's modulus = 82 kPa). These advantages enable DNH to immediately fill the irregular shape of the wound after gelation and remain intact after being squeezed. Swelling tests indicated that DNH had a suitable swelling ratio and maintained its structural integrity after swelling. We evaluated the use of DNH as a moist dressing for full-thickness wound healing in vivo. DNH-treated wounds healed faster, with enhanced blood vessel formation and macrophage polarization than gauze-treated wounds. These findings suggest that DNH not only accelerates wound healing but also improves tissue regeneration. Therefore, DNH may be a suitable moist dressing for wound healing.
作为用于湿性伤口愈合的敷料，透明质酸水凝胶在保持伤口湿润和促进伤口愈合方面发挥着重要作用。然而，现有的水凝胶敷料在凝胶化时间长、机械性能弱和降解快方面存在不足，这增加了治疗期间继发感染的风险。因此，我们开发了一种透明质酸双网络水凝胶（DNH）。与单网络水凝胶（腙和狄尔斯-阿尔德反应型）相比，DNH显示出较短的凝胶化时间（25秒）和较强的机械性能（杨氏模量 = 82 kPa）。这些优点使DNH在凝胶化后能够立即填充伤口的不规则形状，并且在挤压后仍保持完整。肿胀试验表明，DNH具有合适的肿胀率，并且在肿胀后保持其结构完整性。我们评估了DNH作为全层伤口愈合的湿性敷料在体内的应用。与纱布处理的伤口相比，DNH处理的伤口愈合更快，血管形成增强，巨噬细胞极化改善。这些发现表明，DNH不仅能加速伤口愈合，还能促进组织再生。因此，DNH可能是一种适合伤口愈合的湿性敷料。
