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三种鲑科鱼类的异染色质和带型核型。

Heterochromatins and band karyotypes in three species of salmonids.

机构信息

Institute for Animal Breeding and Genetics, Veterinary University, A-1030, Vienna, Austria.

出版信息

Theor Appl Genet. 1988 Jul;76(1):45-53. doi: 10.1007/BF00288830.

DOI:10.1007/BF00288830
PMID:24231981
Abstract

The heterochromatins of rainbow trout (Salmo gairdneri R.), brown trout (Salmo trutta fario L.) and brook trout (Salvelinus fontinalis M.) were characterized by sequential chromomycin A3/distamycin A/DAPI (CDD) and DAPI/actinomycin D (DAPI/AmD) fluorescence. On most biarmed chromosomes, an equilocal localization of prominent DAPI/AmD positive, chromomycin A3 negative, AT-rich blocks at the centromeres were observed in all three species. Band karyotypes of the three species were established. In rainbow trout, several DAPI/AmD positive heterochromatin blocks behaved positive in a silver-staining method. Mitotic and interphase studies proved the presence of inter-individual NOR variation in brown trout. The NORs of brook trout were localized on chromosomes 5, 10, 14, 15 and 29.

摘要

虹鳟鱼(Salmo gairdneri R.)、褐鳟鱼(Salmo trutta fario L.)和溪红点鲑(Salvelinus fontinalis M.)的异染色质通过顺序地用 chromomycin A3/distamycin A/DAPI(CDD)和 DAPI/actinomycin D(DAPI/AmD)荧光染色来进行分析。在大多数臂间染色体上,在这三个物种中,在着丝粒处观察到等区定位的明显的 DAPI/AmD 阳性、chromomycin A3 阴性、富含 AT 的块。建立了这三个物种的带核型。在虹鳟鱼中,几种 DAPI/AmD 阳性异染色质块在银染方法中表现为阳性。有丝分裂和间期研究证明了褐鳟鱼存在个体间 NOR 变异。溪红点鲑的 NOR 位于染色体 5、10、14、15 和 29 上。

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本文引用的文献

1
Localisation of NORs and counterstain-enhanced fluorescence studies in Salmo gairdneri and Salmo trutta (Pisces, Salmonidae).在鲑鱼和鳟鱼(鱼类,鲑科)中 NORs 的定位和反染色增强荧光研究。
Theor Appl Genet. 1986 Feb;71(5):703-7. doi: 10.1007/BF00263267.
2
Q band chromosomal polymorphisms in lake trout (Salvelinus namaycush).湖鳟(Salvelinus namaycush)的Q带染色体多态性。
Genetics. 1982 Jun;101(2):227-34. doi: 10.1093/genetics/101.2.227.
3
Cytogenetic investigations in Austrian bulls and boars.奥地利公牛和公猪的细胞遗传学研究。
棕鳟(Salmo trutta)的罗伯逊易位和 Ag-NOR 多态性的群体分析。
Theor Appl Genet. 1994 Sep;89(1):105-11. doi: 10.1007/BF00226990.
4
Multi-chromosomal location of ribosomal RNA genes and heterochromatin association in brown trout.褐鳟核糖体RNA基因的多染色体定位及与异染色质的关联
Chromosome Res. 1993 May;1(1):63-7. doi: 10.1007/BF00710608.
5
Organization and chromosomal location of the major histone cluster in brown trout, Atlantic salmon and rainbow trout.褐鳟、大西洋鲑和虹鳟主要组蛋白簇的组织与染色体定位。
Chromosoma. 1994 Apr;103(2):147-52. doi: 10.1007/BF00352324.
Zentralbl Veterinarmed A. 1981;28(1):70-5. doi: 10.1111/j.1439-0442.1981.tb01164.x.
4
Counterstain-enhanced chromosome banding.复染增强染色体显带
Hum Genet. 1981;57(1):1-14.
5
An improved silver staining technique for nucleolus organizer regions by using nylon cloth.一种使用尼龙布改进的核仁组织区银染技术。
Jinrui Idengaku Zasshi. 1980 Sep;25(3):229-33. doi: 10.1007/BF01997700.
6
Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method.用保护性胶体显影剂对核仁组织区进行对照银染:一种一步法。
Experientia. 1980 Aug 15;36(8):1014-5. doi: 10.1007/BF01953855.
7
Chromosome banding in salmonid fish: nucleolar organizer regions in Salmo and Salvelinus.鲑科鱼类的染色体显带:鲑属和红点鲑属中的核仁组织区
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