Buell R H, Tremblay G
Cancer Res. 1985 Sep;45(9):4278-84.
This report describes the localization of bound radioactivity as visualized by thaw-mount autoradiography in 35 cases of human breast carcinoma after in vitro incubation with [3H]estradiol. The findings have been compared qualitatively with results of biochemical assays. Twenty-six tumors were considered to be estrogen receptor positive by autoradiographic criteria. Of these, 23 were assayed biochemically; 21 were found to be positive. Specific uptake of radioactivity was observed primarily in neoplastic epithelial cells. The grains were localized mainly over the nuclear region of putative target cells. Within nests of infiltrating carcinoma, positive cells could be identified admixed with negative cells. There were no autoradiographic criteria established for borderline cases. The remaining nine cases were considered negative. In these cases the appearance of tissue exposed only to [3H]estradiol resembled that of tissue incubated with excess unlabeled estradiol. The few grains were randomly scattered with no evidence of nuclear localization. Of these negative cases, two were positive by biochemical assay, four borderline, and three negative. It is concluded that, as assessed in this investigation, estrogen receptor-positive mammary carcinomas may be composed of target and nontarget neoplastic cells. Thaw-mount autoradiography should prove useful in the laboratory investigation of steroid hormone responsiveness of human breast carcinoma.
本报告描述了35例人乳腺癌在与[3H]雌二醇体外孵育后，通过冻融切片放射自显影法观察到的结合放射性的定位情况。研究结果已与生化检测结果进行了定性比较。根据放射自显影标准，26个肿瘤被认为雌激素受体阳性。其中23个进行了生化检测，21个呈阳性。放射性的特异性摄取主要在肿瘤上皮细胞中观察到。颗粒主要定位于假定靶细胞的核区域。在浸润性癌巢内，可识别出阳性细胞与阴性细胞混合存在。对于临界病例，没有建立放射自显影标准。其余9例被认为是阴性。在这些病例中，仅暴露于[3H]雌二醇的组织外观类似于用过量未标记雌二醇孵育的组织。少数颗粒随机散布，没有核定位的证据。在这些阴性病例中，2例生化检测呈阳性，4例临界，3例阴性。结论是，如本研究评估所示，雌激素受体阳性的乳腺癌可能由靶性和非靶性肿瘤细胞组成。冻融切片放射自显影法在人乳腺癌类固醇激素反应性的实验室研究中应会被证明有用。
